Random and Nonrandom Integration of a Polyomavirus DNA Molecule Containing Highly Repetitive Cellular Sequences

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1984 Jun 1

PMID 6327998

Citations 6

Authors

J C Wallenburg

A NEPVEU

P Chartrand

Affiliations

Soon will be listed here.

Abstract

RmI is a circular DNA molecule that consists of a complete polyomavirus genome with an insertion (Ins) of mouse cellular DNA. This polyomavirus genome carries a mutation which renders its replication, but not its transforming ability, temperature sensitive. Ins contains both unique and repetitive cellular DNA sequences. We transfected RmI into rat cells at the permissive and nonpermissive temperatures for replication and isolated clones that had integrated RmI in their genomes. In this paper, we describe detailed mapping of the integrated RmI sequences present in 37 different cell clones. Our results indicated that transfection at the permissive temperature resulted in a random integration pattern, whereas transfection at the nonpermissive temperature resulted in a nonrandom integration pattern. The nonrandom insertions had a preferential length and preferential endpoints. We argue from these results that the nonrandom integration pattern is related to the presence of Ins and that the switch between nonrandom integration and random integration reflects a modification of the integrating substrate. When both are active, the random mechanism dominates the nonrandom mechanism.

Citing Articles

Hotspots of meiotic recombination in the mouse major histocompatibility complex.

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Intermolecular recombination assay for mammalian cells that produces recombinants carrying both homologous and nonhomologous junctions.

Brouillette S, Chartrand P Mol Cell Biol. 1987; 7(6):2248-55.

PMID: 3037354 PMC: 365349. DOI: 10.1128/mcb.7.6.2248-2255.1987.

Linear DNA must have free ends to transform rat cells efficiently.

Gusew N, NEPVEU A, Chartrand P Mol Gen Genet. 1987; 206(1):121-5.

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Integration of a vector containing rodent repetitive elements in the rat genome.

Wallenburg J, NEPVEU A, Chartrand P Nucleic Acids Res. 1987; 15(19):7849-63.

PMID: 2823220 PMC: 306312. DOI: 10.1093/nar/15.19.7849.

Recombinational hotspot specific to female meiosis in the mouse major histocompatibility complex.

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