A Site-specific, Conservative Recombination System Carried by Bacteriophage P1. Mapping the Recombinase Gene Cin and the Cross-over Sites Cix for the Inversion of the C Segment

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1982 Jan 1

PMID 6327269

Citations 41

Authors

S Iida

J Meyer

K E Kennedy

W Arber

Affiliations

Soon will be listed here.

Abstract

The bacteriophage P1 genome carries an invertible C segment consisting of 3-kb unique sequences flanked by 0.6-kb inverted repeats. With insertion and deletion mutants of P1 derivatives the site-specific recombinase gene cin for C inversion) has been mapped adjacent to the C segment and the cix sites (for C inversion cross-over) have been located at the outside ends of the inverted repeats. Inversion of the C segment functions as a biological switch and controls expression of the gene(s) responsible for phage infectivity carried on the C segment. The cin gene product can promote recombination between a 'quasi- cix ' site on plasmid pBR322 and a cix site on P1 DNA. The junctions formed on the resulting co-integrate can also serve as cix sites. This observation implies a potential evolutionary process to bring genes under the control of a biological switch acting by DNA inversion.

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