DNA Methylation Inhibits the Transfecting Activity of Replicative- Form Phi X174 DNA

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1984 Mar 1

PMID 6321756

Citations 8

Authors

R Y Wang

S Shenoy

M Ehrlich

Affiliations

Soon will be listed here.

Abstract

Replacement of virtually all the cytosine residues with 5-methylcytosine residues in the complementary strand of the replicative form (RF) of phi X174 DNA caused a 300- to 500-fold loss in its transfecting activity. Similar results were obtained with analogously methylated M13 RF. Transfection experiments with phi X RF hemimethylated in only part of the molecule, as assessed by analysis with restriction endonucleases, indicated that gene A of phi X, which needs to be nicked at a specific site by the gene A protein for RF replication, was not the main target for this inhibition by DNA methylation. We propose that the loss of transfecting activity was due to hemimethylation of the phi X RF interfering with the processively catalyzed movement of the replication fork.

Citing Articles

Conformation-selective methylation of geminivirus DNA.

Paprotka T, Deuschle K, Metzler V, Jeske H J Virol. 2011; 85(22):12001-12.

PMID: 21835804 PMC: 3209285. DOI: 10.1128/JVI.05567-11.

Perturbation of maintenance and de novo DNA methylation in vitro by UVB (280-340 nm)-induced pyrimidine photodimers.

Becker F, Holton P, Ruchirawat M, Lapeyre J Proc Natl Acad Sci U S A. 1985; 82(18):6055-9.

PMID: 3862117 PMC: 390698. DOI: 10.1073/pnas.82.18.6055.

Cytosine-specific DNA modification interferes with plasmid establishment in Escherichia coli K12: involvement of rglB.

Noyer-Weidner M, Diaz R, REINERS L Mol Gen Genet. 1986; 205(3):469-75.

PMID: 3550384 DOI: 10.1007/BF00338084.

Methylated DNA-binding protein from human placenta recognizes specific methylated sites on several prokaryotic DNAs.

Wang R, Zhang X, Khan R, Zhou Y, Huang L, Ehrlich M Nucleic Acids Res. 1986; 14(24):9843-60.

PMID: 3027666 PMC: 341339. DOI: 10.1093/nar/14.24.9843.

Hydrolysis by restriction endonucleases at their DNA recognition sequences substituted with mismatched base pairs.

Shenoy S, Daigle K, Ehrlich K, GEHRKE C, Ehrlich M Nucleic Acids Res. 1986; 14(11):4407-20.

PMID: 3012472 PMC: 311455.

References

Baas P, Jansz H . Asymmetric information transfer during phi X174 DNA replication. J Mol Biol. 1972; 63(3):557-68. DOI: 10.1016/0022-2836(72)90447-0. View

Mermod J, Bourgeois S, Defer N, Crepin M . Demethylation and expression of murine mammary tumor proviruses in mouse thymoma cell lines. Proc Natl Acad Sci U S A. 1983; 80(1):110-4. PMC: 393319. DOI: 10.1073/pnas.80.1.110. View

Henner W, Kleber I, Benzinger R . Transfection of Escherichia coli spheroplasts. 3. Facilitation of transfection and stabilization of spheroplasts by different basic polymers. J Virol. 1973; 12(4):741-7. PMC: 356692. DOI: 10.1128/JVI.12.4.741-747.1973. View

Ehrlich M, Ehrlich K, Mayo J . Unusual properties of the DNA from Xanthomonas phage XP-12 in which 5-methylcytosine completely replaces cytosine. Biochim Biophys Acta. 1975; 395(2):109-19. DOI: 10.1016/0005-2787(75)90149-5. View

SANGER F, Air G, Barrell B, Brown N, Coulson A, Fiddes C . Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977; 265(5596):687-95. DOI: 10.1038/265687a0. View

Eisenberg S, Griffith J, Kornberg A . phiX174 cistron A protein is a multifunctional enzyme in DNA replication. Proc Natl Acad Sci U S A. 1977; 74(8):3198-202. PMC: 431495. DOI: 10.1073/pnas.74.8.3198. View

VOVIS G, Lacks S . Complementary action of restriction enzymes endo R-DpnI and Endo R-DpnII on bacteriophage f1 DNA. J Mol Biol. 1977; 115(3):525-38. DOI: 10.1016/0022-2836(77)90169-3. View

Wickner S . DNA replication proteins of Escherichia coli. Annu Rev Biochem. 1978; 47:1163-91. DOI: 10.1146/annurev.bi.47.070178.005503. View

Bale A, dAlarcao M, Marinus M . Characterization of DNA adenine methylation mutants of Escherichia coli K12. Mutat Res. 1979; 59(2):157-65. DOI: 10.1016/0027-5107(79)90153-2. View

10.

Kunkel T, Loeb L . On the fidelity of DNA replication. Effect of divalent metal ion activators and deoxyrionucleoside triphosphate pools on in vitro mutagenesis. J Biol Chem. 1979; 254(13):5718-25. View

11.

Warner H, Thompson R, Mozer T, Duncan B . The properties of a bacteriophage T5 mutant unable to induce deoxyuridine 5'-triphosphate nucleotidohydrolase. Synthesis of uracil-containing T5 deoxyribonucleic acid. J Biol Chem. 1979; 254(16):7534-9. View

12.

Kan N, Lautenberger J, Edgell M, HUTCHISON 3rd C . The nucleotide sequence recognized by the Escherichia coli K12 restriction and modification enzymes. J Mol Biol. 1979; 130(2):191-209. DOI: 10.1016/0022-2836(79)90426-1. View

13.

Hattman S, Gribbin C, HUTCHISON 3rd C . In vivo methylation of bacteriophage phi X174 DNA. J Virol. 1979; 32(3):845-51. PMC: 525933. DOI: 10.1128/JVI.32.3.845-851.1979. View

14.

Baas P, van Teeffelen H, Teertstra W, Jansz H, Veeneman G, van der Marel G . Restoration of the biological activity of in vitro synthesized phi X DNA by transfection of ung- spheroplasts or dUTPase treatment. FEBS Lett. 1980; 110(1):15-20. DOI: 10.1016/0014-5793(80)80012-3. View

15.

Yoo O, Agarwal K . Isolation and characterization of two proteins possessing Hpa II methylase activity. J Biol Chem. 1980; 255(13):6445-9. View

16.

van Mansfeld A, Langeveld S, Baas P, Jansz H, van der Marel G, Veeneman G . Recognition sequence of bacteriophage phi X174 gene A protein--an initiator of DNA replication. Nature. 1980; 288(5791):561-6. DOI: 10.1038/288561a0. View

17.

KUO K, McCune R, GEHRKE C, Midgett R, Ehrlich M . Quantitative reversed-phase high performance liquid chromatographic determination of major and modified deoxyribonucleosides in DNA. Nucleic Acids Res. 1980; 8(20):4763-76. PMC: 324386. DOI: 10.1093/nar/8.20.4763. View

18.

Wang R, GEHRKE C, Ehrlich M . Comparison of bisulfite modification of 5-methyldeoxycytidine and deoxycytidine residues. Nucleic Acids Res. 1980; 8(20):4777-90. PMC: 324387. DOI: 10.1093/nar/8.20.4777. View

19.

Caradonna S, Cheng Y . The role of deoxyuridine triphosphate nucleotidohydrolase, uracil-DNA glycosylase, and DNA polymerase alpha in the metabolism of FUdR in human tumor cells. Mol Pharmacol. 1980; 18(3):513-20. View

20.

Arai N, Polder L, Akai K, Kornberg A . Replication of phi X174 DNA with purified enzymes. II. Multiplication of the duplex form by coupling of continuous and discontinuous synthetic pathways. J Biol Chem. 1981; 256(10):5239-46. View