Retrovirus Transduction: Segregation of the Viral Transforming Function and the Herpes Simplex Virus Tk Gene in Infectious Friend Spleen Focus-forming Virus Thymidine Kinase Vectors

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1983 Dec 1

PMID 6318088

Citations 20

Authors

A L Joyner

A Bernstein

Affiliations

Soon will be listed here.

Abstract

A series of deletions and insertions utilizing the herpesvirus thymidine kinase gene (tk) were constructed in the murine retrovirus Friend spleen focus-forming virus (SFFV). In all cases, the coding region for the SFFV-specific glycoprotein (gp55), which is implicated in erythroleukemic transformation, was left intact. These SFFV-TK and SFFV deletion vectors were analyzed for expression of tk and gp55 after DNA-mediated gene transfer. In addition, virus rescued by cotransfection of these vectors with Moloney murine leukemia virus was analyzed for infectious TK-transducing virus, gp55 expression, and erythroleukemia-inducing ability. The experiments demonstrated that deletions or insertions within the intron for the gp55 env gene can interfere with expression of gp55 after both DNA-mediated gene transfer and virus infection. In contrast, the gene transfer efficiency of the tk gene was unaffected in the SFFV-TK vectors, and high-titer infectious TK virus could be recovered. Revertant viruses capable of inducing erythroleukemia and expressing gp55 were generated after cotransfection of the SFFV-TK vectors with murine leukemia virus. The revertant viruses lost both tk sequences and the ability to transduce TK- fibroblasts to a TK+ phenotype. These experiments demonstrate that segregation of the TK and erythroleukemia functions can occur in retrovirus vectors which initially carry both markers.

Citing Articles

Selectable retrovirus vectors encoding Friend virus gp55 or erythropoietin induce polycythemia with different phenotypic expression and disease progression.

Ahlers N, Hunt N, Just U, Laker C, Ostertag W, Nowock J J Virol. 1994; 68(11):7235-43.

PMID: 7933106 PMC: 237163. DOI: 10.1128/JVI.68.11.7235-7243.1994.

Retrovirus transduction: generation of infectious retroviruses expressing dominant and selectable genes is associated with in vivo recombination and deletion events.

Joyner A, Bernstein A Mol Cell Biol. 1983; 3(12):2180-90.

PMID: 6318087 PMC: 370089. DOI: 10.1128/mcb.3.12.2180-2190.1983.

Role of intron-contained sequences in formation of moloney murine leukemia virus env mRNA.

Hwang L, Park J, Gilboa E Mol Cell Biol. 1984; 4(11):2289-97.

PMID: 6096692 PMC: 369057. DOI: 10.1128/mcb.4.11.2289-2297.1984.

Retrovirus vectors and their uses in molecular biology.

Gilboa E Bioessays. 1986; 5(6):252-7.

PMID: 3551938 PMC: 7161754. DOI: 10.1002/bies.950050605.

Self-inactivating retroviral vectors designed for transfer of whole genes into mammalian cells.

Yu S, von Ruden T, Kantoff P, Garber C, Seiberg M, Ruther U Proc Natl Acad Sci U S A. 1986; 83(10):3194-8.

PMID: 3458176 PMC: 323479. DOI: 10.1073/pnas.83.10.3194.

References

Boyer H . A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969; 41(3):459-72. DOI: 10.1016/0022-2836(69)90288-5. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Rowe W, PUGH W, Hartley J . Plaque assay techniques for murine leukemia viruses. Virology. 1970; 42(4):1136-9. DOI: 10.1016/0042-6822(70)90362-4. View

Graham F, Van Der Eb A . A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973; 52(2):456-67. DOI: 10.1016/0042-6822(73)90341-3. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Racevskis J, Koch G . Viral protein synthesis in Friend erythroleukemia cell lines. J Virol. 1977; 21(1):328-37. PMC: 353819. DOI: 10.1128/JVI.21.1.328-337.1977. View

Bernstein A, Mak T, Stephenson J . The Friend virus genome: evidence for the stable association of MuLV sequences and sequences involved in erythroleukemic transformation. Cell. 1977; 12(1):287-94. DOI: 10.1016/0092-8674(77)90206-9. View

Brugge J, Erikson R . Identification of a transformation-specific antigen induced by an avian sarcoma virus. Nature. 1977; 269(5626):346-8. DOI: 10.1038/269346a0. View

Wigler M, Sweet R, Sim G, Wold B, Pellicer A, Lacy E . Transformation of mammalian cells with genes from procaryotes and eucaryotes. Cell. 1979; 16(4):777-85. DOI: 10.1016/0092-8674(79)90093-x. View

10.

Dagert M, Ehrlich S . Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979; 6(1):23-8. DOI: 10.1016/0378-1119(79)90082-9. View

11.

Wahl G, Stern M, Stark G . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979; 76(8):3683-7. PMC: 383897. DOI: 10.1073/pnas.76.8.3683. View

12.

Enquist L, Woude G, Wagner M, SMILEY J, Summers W . Construction and characterization of a recombinant plasmid encoding the gene for the thymidine kinase of Herpes simplex type 1 virus. Gene. 1979; 7(3-4):335-42. DOI: 10.1016/0378-1119(79)90052-0. View

13.

Ruscetti S, Troxler D, Linemeyer D, Scolnick E . Three laboratory strains of spleen focus-forming virus: comparison of their genomes and translational products. J Virol. 1980; 33(1):140-51. PMC: 288532. DOI: 10.1128/JVI.33.1.140-151.1980. View

14.

Bosselman R, van Griensven L, Vogt M, Verma I . Genome organization of retroviruses IX. Analysis of the genomes of Friend spleen focus-forming (F-SFFV) and helper murine leukemia viruses by heteroduplex-formation. Virology. 1980; 102(1):234-9. DOI: 10.1016/0042-6822(80)90088-4. View

15.

Edgell M, Polsky F . Use of preparative gel electrophoresis for DNA fragment isolation. Methods Enzymol. 1980; 65(1):319-27. DOI: 10.1016/s0076-6879(80)65042-3. View

16.

Macdonald M, Reynolds Jr F, Van de Ven W, Stephenson J, Mak T, Bernstein A . Anemia- and polycythemia-inducing isolates of Friend spleen focus-forming virus. Biological and molecular evidence for two distinct viral genomes. J Exp Med. 1980; 151(6):1477-92. PMC: 2185875. DOI: 10.1084/jem.151.6.1477. View

17.

Bilello J, Colletta G, Warnecke G, Koch G, Frisby D, Pragnell I . Analysis of the expression of spleen focus-forming virus (SFFV)-related RNA and gp55, a Friend and Rauscher virus-specific protein. Virology. 1980; 107(2):331-44. DOI: 10.1016/0042-6822(80)90301-3. View

18.

Linemeyer D, Ruscetti S, Scolnick E, Evans L, Duesberg P . Biological activity of the spleen focus-forming virus is encoded by a molecularly cloned subgenomic fragment of spleen focus-forming virus DNA. Proc Natl Acad Sci U S A. 1981; 78(3):1401-5. PMC: 319138. DOI: 10.1073/pnas.78.3.1401. View

19.

Mager D, Mak T, Bernstein A . Quantitative colony method for tumorigenic cells transformed by two distinct strains of Friend leukemia virus. Proc Natl Acad Sci U S A. 1981; 78(3):1703-7. PMC: 319201. DOI: 10.1073/pnas.78.3.1703. View

20.

Wendling F, Moreau-Gachelin F, Tambourin P . Emergence of tumorigenic cells during the course of Friend virus leukemias. Proc Natl Acad Sci U S A. 1981; 78(6):3614-8. PMC: 319621. DOI: 10.1073/pnas.78.6.3614. View