Simple Screening Method for Isolation of Penicillin Acylase-producing Bacteria

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1983 Nov 1

PMID 6316854

Citations 5

Authors

V Meevootisom

P Somsuk

R Prachaktam

T W Flegel

Affiliations

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Abstract

A new screening method for bacteria capable of producing penicillin acylase is described. The method is based on the use of Serratia marcescens sensitive to 6-aminopenicillanic acid but comparatively resistant to benzylpenicillin. It is simple, quite specific, and requires no special equipment. It can also be used to screen for phenoxymethylpenicillin acylase activity. We also suggest an acidimetric method for rapid detection of cloned genes in genetic engineering studies of penicillin acylase.

Citing Articles

Protein engineering of penicillin acylase.

Tishkov V, Savin S, Yasnaya A Acta Naturae. 2012; 2(3):47-61.

PMID: 22649651 PMC: 3347563.

Developing an extended genomic engineering approach based on recombineering to knock-in heterologous genes to Escherichia coli genome.

Sukhija K, Pyne M, Ali S, Orr V, Abedi D, Moo-Young M Mol Biotechnol. 2011; 51(2):109-18.

PMID: 21826554 DOI: 10.1007/s12033-011-9442-2.

Chaperone-mediated folding and maturation of the penicillin acylase precursor in the cytoplasm of Escherichia coli.

Xu Y, Weng C, Narayanan N, Hsieh M, Anderson W, Scharer J Appl Environ Microbiol. 2005; 71(10):6247-53.

PMID: 16204545 PMC: 1265976. DOI: 10.1128/AEM.71.10.6247-6253.2005.

Molecular cloning of the penicillin G acylase gene from Arthrobacter viscosus.

Ohashi H, Katsuta Y, Hashizume T, Abe S, Kajiura H, Hattori H Appl Environ Microbiol. 1988; 54(11):2603-7.

PMID: 3214149 PMC: 204342. DOI: 10.1128/aem.54.11.2603-2607.1988.

Expression of the Arthrobacter viscosus penicillin G acylase gene in Escherichia coli and Bacillus subtilis.

Ohashi H, Katsuta Y, Nagashima M, Kamei T, Yano M Appl Environ Microbiol. 1989; 55(6):1351-6.

PMID: 2504107 PMC: 202870. DOI: 10.1128/aem.55.6.1351-1356.1989.

References

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