Cloning and Characterisation of NifLA Regulatory Mutations from Klebsiella Pneumoniae

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1983 Jan 1

PMID 6314096

Citations 16

Authors

M Filser

M Merrick

F Cannon

Affiliations

Soon will be listed here.

Abstract

A total of nine regulatory mutations in the nifLA operon of Klebsiella pneumoniae were cloned in the high copy-number plasmid vector pACYC184. The regulatory phenotypes of the resultant clones were then correlated with their restriction maps and their ability to synthesise nifL and nifA polypeptides in vivo. One mutation, nifL2401, was identified as a 400 bp. deletion within the nifL gene. This mutation was non-polar and caused a Nif+ phenotype which showed escape from repression by oxygen and low levels of fixed nitrogen. Identification of this deletion allows the first definitive allocation of a mutation with this phenotype to the nifL gene and provides further evidence for the role of the nifL gene product in nif-specific repression.

Citing Articles

Iron is required to relieve inhibitory effects on NifL on transcriptional activation by NifA in Klebsiella pneumoniae.

Schmitz R, He L, Kustu S J Bacteriol. 1996; 178(15):4679-87.

PMID: 8755900 PMC: 178239. DOI: 10.1128/jb.178.15.4679-4687.1996.

Transcription termination within the regulatory nifLA operon of Klebsiella pneumoniae.

Govantes F, Santero E Mol Gen Genet. 1996; 250(4):447-54.

PMID: 8602162

Regulation of nif gene expression in Enterobacter agglomerans: nucleotide sequence of the nifLA operon and influence of temperature and ammonium on its transcription.

Siddavattam D, Steibl H, Kreutzer R, Klingmuller W Mol Gen Genet. 1995; 249(6):629-36.

PMID: 8544828 DOI: 10.1007/BF00418032.

Characterisation of mutations in the Klebsiella pneumoniae nitrogen fixation regulatory gene nifL which impair oxygen regulation.

Sidoti C, HARWOOD G, Ackerman R, Coppard J, Merrick M Arch Microbiol. 1993; 159(3):276-81.

PMID: 8481091 DOI: 10.1007/BF00248484.

In vitro activity of NifL, a signal transduction protein for biological nitrogen fixation.

Lee H, Narberhaus F, Kustu S J Bacteriol. 1993; 175(23):7683-8.

PMID: 8244938 PMC: 206926. DOI: 10.1128/jb.175.23.7683-7688.1993.

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