Intracistronic Complementation in the Simian Virus 40 A Gene

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1983 Oct 1

PMID 6312452

Citations 22

Authors

J Tornow

C N Cole

Affiliations

Soon will be listed here.

Abstract

A set of eight simian virus 40 mutants was constructed with lesions in the A gene, which encodes the large tumor (T) antigen. These mutants have small deletions (3-20 base pairs) at either 0.497, 0.288, or 0.243 map units. Mutants having both in-phase and frameshift mutations at each site were isolated. Neither plaque formation nor replication of the mutant DNAs could be detected after transfection of monkey kidney cells. Another nonviable mutant, dlA2459, had a 14-base-pair deletion at 0.193 map unit and was positive for viral DNA replication. Each of the eight mutants were tested for ability to form plaques after cotransfection with dlA2459 DNA. The four mutants that had in-phase deletions were able to complement dlA2459. The other four, which had frameshift deletions, did not. No plaques were formed after cotransfection of cells with any other pair of group A mutants. This suggests that the defect in dlA2459 defines a distinct functional domain of simian virus 40 T antigen.

Citing Articles

Interactions required for binding of simian virus 40 T antigen to the viral origin and molecular modeling of initial assembly events.

Reese D, Sreekumar K, Bullock P J Virol. 2004; 78(6):2921-34.

PMID: 14990710 PMC: 353773. DOI: 10.1128/jvi.78.6.2921-2934.2004.

Regions and activities of simian virus 40 T antigen that cooperate with an activated ras oncogene in transforming primary rat embryo fibroblasts.

Beachy T, Cole S, Cavender J, Tevethia M J Virol. 2002; 76(7):3145-57.

PMID: 11884539 PMC: 136032. DOI: 10.1128/jvi.76.7.3145-3157.2002.

Database of mutations that alter the large tumor antigen in simian virus 40.

Kierstead T, Pipas J Nucleic Acids Res. 1996; 24(1):125-6.

PMID: 8594559 PMC: 145579. DOI: 10.1093/nar/24.1.125.

Efficient transcriptional activation of many simple modular promoters by simian virus 40 large T antigen.

Rice P, Cole C J Virol. 1993; 67(11):6689-97.

PMID: 8411371 PMC: 238108. DOI: 10.1128/JVI.67.11.6689-6697.1993.

Simian virus 40 large T-antigen point mutants that are defective in viral DNA replication but competent in oncogenic transformation.

Manos M, Gluzman Y Mol Cell Biol. 1984; 4(6):1125-33.

PMID: 6330530 PMC: 368881. DOI: 10.1128/mcb.4.6.1125-1133.1984.

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