RNAs Transcribed from a 3.6-kilobase SmaI Fragment of the Short Unique Region of the Herpes Simplex Virus Type 1 Genome

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1983 Nov 1

PMID 6312102

Citations 9

Authors

K Ikura

J L Betz

J R SADLER

L I PIZER

Affiliations

Soon will be listed here.

Abstract

A 3.6-kilobase (kb) SmaI subclone of the BamHI J fragment of herpes simplex virus type 1 (KOS) DNA was utilized to characterize the mRNAs transcribed from the genome segment (0.91 to 0.93 map units) that encodes glycoprotein D mRNA. RNA blotting demonstrated two major RNA species of 2.3 and 1.5 kb. 5' and 3' mapping with 32P-end-labeled DNA fragments indicated that these RNAs are a nested set, each having its own promoter and 3' terminus. Less abundant RNA species with discrete 5' ends were also observed. Precise 5' mapping and sequence data located the initiation sites and demonstrated TATA boxes, CAT boxes, and AC-rich regions in the appropriate positions. 3' mapping located a common end for both mRNAs, but the 2.3-kb mRNA was reduced in size by splicing at a point near the RNA terminus. In vitro runoff transcription experiments confirmed the location of the two promoters and showed that an uninfected cell extract initiated faithfully at both sites. Despite the similarities in DNA structure and the apparent equal efficiency of promoter utilization in vitro, the 2.3-kb mRNA appeared in the cytoplasm early (1 h) after infection, whereas the 1.5-kb mRNA was delayed until 3 h after infection.

Citing Articles

Mapping of the transcriptional initiation site of the herpes simplex virus type 1 ICP8 gene in infected and transfected cells.

Su L, Knipe D J Virol. 1987; 61(2):615-20.

PMID: 3027391 PMC: 253992. DOI: 10.1128/JVI.61.2.615-620.1987.

Regulation of transcription in vitro from herpes simplex virus genes.

PIZER L, Tedder D, Betz J, WILCOX K, Beard P J Virol. 1986; 60(3):950-9.

PMID: 3023683 PMC: 253333. DOI: 10.1128/JVI.60.3.950-959.1986.

Herpes simplex virus immediate early infected-cell polypeptide 4 binds to DNA and promotes transcription.

Beard P, Faber S, WILCOX K, PIZER L Proc Natl Acad Sci U S A. 1986; 83(11):4016-20.

PMID: 3012542 PMC: 323656. DOI: 10.1073/pnas.83.11.4016.

Detailed analysis of the mRNAs mapping in the short unique region of herpes simplex virus type 1.

Rixon F, McGeoch D Nucleic Acids Res. 1985; 13(3):953-73.

PMID: 2987814 PMC: 341045. DOI: 10.1093/nar/13.3.953.

In vitro transcription of herpes simplex virus genes: identification of a new initiation site and second intervening sequence in the immediate-early RNA-5 gene.

Fontichiaro K, Beck T, MILLETTE R J Virol. 1985; 53(1):235-42.

PMID: 2981342 PMC: 255020. DOI: 10.1128/JVI.53.1.235-242.1985.

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