Incorporation of Mannose 6-phosphate Receptors into Liposomes. Receptor Topography and Binding of Alpha-mannosidase

Overview

Journal Biochem J

Specialty Biochemistry

Date 1983 Aug 15

PMID 6311183

Authors

C H CAMPBELL

A L Miller

L H Rome

Affiliations

Soon will be listed here.

Abstract

A receptor that binds the lysosomal enzyme alpha-mannosidase via mannose 6-phosphate moieties (mannose 6-phosphate receptor) was purified from Swarm-rat chondrosarcoma and bovine liver microsomal membranes. Receptor-reconstituted liposomes were prepared by dialysis of taurodeoxycholate-dispersed lipids with purified mannose 6-phosphate receptor. Liposomes appeared by electron microscopy as 60-120 nm unilamellar vesicles. Receptor-reconstituted liposomes retained the ability to bind alpha-mannosidase specifically. Binding was saturable with an apparent Kd of 1 nM and was competitively inhibited by mannose 6-phosphate (Ki 2mM). Liposomes containing entrapped 125I-bovine serum albumin were used to demonstrate that treatment with 0.045% taurodeoxycholate rendered liposomes permeable to macromolecules without solubilizing the membrane. Receptor orientation in the liposome membrane was established by measuring binding of ligand to intact and detergent-treated liposomes. Unlike coated vesicles, which contain cryptic mannose 6-phosphate receptors [Campbell, Fine, Squicciarini & Rome (1983) J. Biol. Chem. 258, 2526-2533], treatment of liposomes with detergent revealed no additional cryptic binding sites. In addition, treatment of liposomes with 0.75% trypsin abolished total receptor binding activity. The results suggest that the receptor is inserted with its binding site facing the outside of the liposome.

References

Gorden P, Carpentier J, Cohen S, Orci L . Epidermal growth factor: morphological demonstration of binding, internalization, and lysosomal association in human fibroblasts. Proc Natl Acad Sci U S A. 1978; 75(10):5025-9. PMC: 336255. DOI: 10.1073/pnas.75.10.5025. View

Steinman R, Mellman I, Muller W, COHN Z . Endocytosis and the recycling of plasma membrane. J Cell Biol. 1983; 96(1):1-27. PMC: 2112240. DOI: 10.1083/jcb.96.1.1. View

Rome L, Garvin A, Allietta M, Neufeld E . Two species of lysosomal organelles in cultured human fibroblasts. Cell. 1979; 17(1):143-53. DOI: 10.1016/0092-8674(79)90302-7. View

Renart J, Reiser J, Stark G . Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure. Proc Natl Acad Sci U S A. 1979; 76(7):3116-20. PMC: 383774. DOI: 10.1073/pnas.76.7.3116. View

Willingham M, Maxfield F, Pastan I . alpha 2 Macroglobulin binding to the plasma membrane of cultured fibroblasts. Diffuse binding followed by clustering in coated regions. J Cell Biol. 1979; 82(3):614-25. PMC: 2110490. DOI: 10.1083/jcb.82.3.614. View

Eimerl S, Neufeld G, Korner M, Schramm M . Functional implantation of a solubilized beta-adrenergic receptor in the membrane of a cell. Proc Natl Acad Sci U S A. 1980; 77(2):760-4. PMC: 348360. DOI: 10.1073/pnas.77.2.760. View

Rome L, Miller J . Butanedione treatment reduces receptor binding of a lysosomal enzyme to cells and membranes. Biochem Biophys Res Commun. 1980; 92(3):986-93. DOI: 10.1016/0006-291x(80)90799-8. View

Alwine J, Kemp D, Parker B, Reiser J, Renart J, Stark G . Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol. 1979; 68:220-42. DOI: 10.1016/0076-6879(79)68017-5. View

Fischer H, Gonzalez-Noriega A, Sly W . Beta-glucuronidase binding to human fibroblast membrane receptors. J Biol Chem. 1980; 255(11):5069-74. View

10.

Wall D, Wilson G, Hubbard A . The galactose-specific recognition system of mammalian liver: the route of ligand internalization in rat hepatocytes. Cell. 1980; 21(1):79-93. DOI: 10.1016/0092-8674(80)90116-6. View

11.

Baumann H, Hou E, Doyle D . Insertion of biologically active membrane proteins from rat liver into the plasma membrane of mouse fibroblasts. J Biol Chem. 1980; 255(20):10001-12. View

12.

Fischer H, Gonzalez-Noriega A, Sly W, Morre D . Phosphomannosyl-enzyme receptors in rat liver. Subcellular distribution and role in intracellular transport of lysosomal enzymes. J Biol Chem. 1980; 255(20):9608-15. View

13.

Lake J, Kahan L . Ribosomal proteins S5, S11, S13 and S19 localized by electron microscopy of antibody-labeled subunits. J Mol Biol. 1975; 99(4):631-44. DOI: 10.1016/s0022-2836(75)80177-x. View

14.

Valentine R, SHAPIRO B, Stadtman E . Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli. Biochemistry. 1968; 7(6):2143-52. DOI: 10.1021/bi00846a017. View

15.

Freeze H, Miller A, Kaplan A . Acid hydrolases from Dictyostelium discoideum contain phosphomannosyl recognition markers. J Biol Chem. 1980; 255(23):11081-4. View

16.

Anderson R, Goldstein J, Brown M . Localization of low density lipoprotein receptors on plasma membrane of normal human fibroblasts and their absence in cells from a familial hypercholesterolemia homozygote. Proc Natl Acad Sci U S A. 1976; 73(7):2434-8. PMC: 430596. DOI: 10.1073/pnas.73.7.2434. View

17.

Maxfield F, Schlessinger J, Shechter Y, Pastan I, Willingham M . Collection of insulin, EGF and alpha2-macroglobulin in the same patches on the surface of cultured fibroblasts and common internalization. Cell. 1978; 14(4):805-10. DOI: 10.1016/0092-8674(78)90336-7. View

18.

Markwell M, Fox C . Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril. Biochemistry. 1978; 17(22):4807-17. DOI: 10.1021/bi00615a031. View

19.

Hasilik A, Klein U, Waheed A, Strecker G, von Figura K . Phosphorylated oligosaccharides in lysosomal enzymes: identification of alpha-N-acetylglucosamine(1)phospho(6)mannose diester groups. Proc Natl Acad Sci U S A. 1980; 77(12):7074-8. PMC: 350443. DOI: 10.1073/pnas.77.12.7074. View

20.

Sahagian G, Distler J, JOURDIAN G . Characterization of a membrane-associated receptor from bovine liver that binds phosphomannosyl residues of bovine testicular beta-galactosidase. Proc Natl Acad Sci U S A. 1981; 78(7):4289-93. PMC: 319775. DOI: 10.1073/pnas.78.7.4289. View