Interaction of Calcium Ions and Salivary Acidic Proline-rich Proteins with Hydroxyapatite. A Possible Aspect of Inhibition of Hydroxyapatite Formation

Overview

Journal Biochem J

Specialty Biochemistry

Date 1983 Jul 1

PMID 6311157

Citations 1

Authors

A Bennick

D Kells

G Madapallimattam

Affiliations

Soon will be listed here.

Abstract

The relationship between Ca2+- and hydroxyapatite-binding sites in salivary acidic proline-rich phosphoproteins A and C was investigated. Coating of hydroxyapatite with protein before adsorption had no effect on Ca2+ binding to the mineral, but simultaneous adsorption of Ca+ and protein to hydroxyapatite caused additional Ca2+ binding to the solid. The additional amount of Ca2+ adsorbed, measured in mol of Ca2+/mol of protein adsorbed to hydroxyapatite, was approx. 2 for protein C, 4 for protein A, 9 for the N-terminal tryptic peptide and 2 for dephosphorylated protein A. It is suggested that the ability of the proteins to inhibit hydroxyapatite formation is related to the binding of the proteins to crystal growth sites on the mineral, which prevents access of Ca2+ from the surrounding liquid.

Citing Articles

Differentiation and interaction of secretory immunoglobulin A and a calcium-dependent parotid agglutinin for several bacterial strains.

Rundegren J, Arnold R Infect Immun. 1987; 55(2):288-92.

PMID: 3100447 PMC: 260323. DOI: 10.1128/iai.55.2.288-292.1987.

References

Bennick A, CONNELL G . Purification and partial characterization of four proteins from human parotid saliva. Biochem J. 1971; 123(3):455-64. PMC: 1176978. DOI: 10.1042/bj1230455. View

Meyer J, Nancollas G . The influence of multidentate organic phosphonates on the crystal growth of hydroxyapatite. Calcif Tissue Res. 1973; 13(4):295-303. DOI: 10.1007/BF02015419. View

Bennick A . Chemical and physical characteristics of a phosphoprotein from human parotid saliva. Biochem J. 1975; 145(3):557-67. PMC: 1165257. DOI: 10.1042/bj1450557. View

Zahradnik R, Moreno E, Burke E . Effect of salivary pellicle on enamel subsurface demineralization in vitro. J Dent Res. 1976; 55(4):664-70. DOI: 10.1177/00220345760550042101. View

Bennick A . Chemical and physical characterization of a phosphoprotein, Protein C, from human saliva and comparison with a related protein A. Biochem J. 1977; 163(2):229-39. PMC: 1164688. DOI: 10.1042/bj1630229. View

Bennick A, Cannon M . Quantitative study of the interaction of salivary acidic proline-rich proteins with hydroxyapatite. Caries Res. 1978; 12(3):159-69. DOI: 10.1159/000260326. View

Rolla G, Bowen W . Surface adsorption of fluoride and ionic exchange reactions on hydroxyapatite. Acta Odontol Scand. 1978; 36(4):219-24. DOI: 10.3109/00016357809004671. View

Hay D, Moreno E . Differential adsorption and chemical affinities of proteins for apatitic surfaces. J Dent Res. 1979; 58(Spec Issue B):930-42. DOI: 10.1177/00220345790580024701. View

Wong R, Hofmann T, Bennick A . The complete primary structure of a proline-rich phosphoprotein from human saliva. J Biol Chem. 1979; 254(11):4800-8. View

10.

Moreno E, Varughese K, Hay D . Effect of human salivary proteins on the precipitation kinetics of calcium phosphate. Calcif Tissue Int. 1979; 28(1):7-16. DOI: 10.1007/BF02441212. View

11.

Bennick A, Cannon M, Madapallimattam G . The nature of the hydroxyapatite-binding site in salivary acidic proline-rich proteins. Biochem J. 1979; 183(1):115-26. PMC: 1161479. DOI: 10.1042/bj1830115. View

12.

Wong R, Bennick A . The primary structure of a salivary calcium-binding proline-rich phosphoprotein (protein C), a possible precursor of a related salivary protein A. J Biol Chem. 1980; 255(12):5943-8. View

13.

Bennick A, Mclaughlin A, Grey A, Madapallimattam G . The location and nature of calcium-binding sites in salivary acidic proline-rich phosphoproteins. J Biol Chem. 1981; 256(10):4741-6. View

14.

Pearce E . Ion displacement following the adsorption of anionic macromolecules on hydroxyapatite. Calcif Tissue Int. 1981; 33(4):395-402. DOI: 10.1007/BF02409462. View

15.

Bennick A, Chau G, GOODLIN R, Abrams S, Tustian D, Madapallimattam G . The role of human salivary acidic proline-rich proteins in the formation of acquired dental pellicle in vivo and their fate after adsorption to the human enamel surface. Arch Oral Biol. 1983; 28(1):19-27. DOI: 10.1016/0003-9969(83)90022-5. View

16.

BARTLETT G . Phosphorus assay in column chromatography. J Biol Chem. 1959; 234(3):466-8. View

17.

Garen A, Levinthal C . A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase. Biochim Biophys Acta. 1960; 38:470-83. DOI: 10.1016/0006-3002(60)91282-8. View