Essential Structure of E. Coli Promoter: Effect of Spacer Length Between the Two Consensus Sequences on Promoter Function

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1983 Sep 10

PMID 6310517

Citations 47

Authors

T Aoyama

M TAKANAMI

E Ohtsuka

Y Taniyama

R Marumoto

H Sato

M Ikehara

Affiliations

Soon will be listed here.

Abstract

A promoter with the consensus sequence(TTGACA and TATAAT) at -35 and -10 regions was constructed, and the distance between the two consensus sequences was independently altered at two restriction sites. In an in vitro transcription system, a maximum activity was observed at the spacer length of 17 base-pairs regardless of the sites of space adjustment. Evidence was also provided that the start point of transcription was fixed by the consensus sequence at the -10 region.

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