In Vitro DNA Synthesis by an Alpha-like DNA Polymerase Bound to Replicating Simian Virus 40 Chromosomes

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1983 Oct 1

PMID 6310151

Citations 2

Authors

M A Waqar

M J Evans

J F Burke

Y Tsubota

M J Plummer

J A Huberman

Affiliations

Soon will be listed here.

Abstract

Simian virus 40 chromosomes carry out replicative DNA synthesis in vitro which is sensitive to aphidicolin and to N-ethylmaleimide, resistant to 2',3'-dideoxythymidine-5'-triphosphate, and proportional to the amount of chromosome-associated alpha-like polymerase. Thus, an alpha-like DNA polymerase (alpha polymerase or delta polymerase) is responsible for in vitro DNA synthesis.

Citing Articles

Monoclonal antibodies as probes for a function of large T antigen during the elongation process of simian virus 40 DNA replication.

Wiekowski M, Droge P, Stahl H J Virol. 1987; 61(2):411-8.

PMID: 3027371 PMC: 253964. DOI: 10.1128/JVI.61.2.411-418.1987.

Initiation of simian virus 40 DNA replication in vitro: aphidicolin causes accumulation of early-replicating intermediates and allows determination of the initial direction of DNA synthesis.

Decker R, Yamaguchi M, Possenti R, DePamphilis M Mol Cell Biol. 1986; 6(11):3815-25.

PMID: 3025613 PMC: 367143. DOI: 10.1128/mcb.6.11.3815-3825.1986.

References

Gourlie B, Miwa M, Pigiet V, Sugimura T, Malik N, Smulson M . Polyoma virus minichromosomes: poly ADP-ribosylation of associated chromatin proteins. J Virol. 1983; 45(2):600-6. PMC: 256454. DOI: 10.1128/JVI.45.2.600-606.1983. View

Gourlie B, Pigiet V . Polyoma virus minichromosomes: characterization of the products of in vitro DNA synthesis. J Virol. 1983; 45(2):585-93. PMC: 256452. DOI: 10.1128/JVI.45.2.585-593.1983. View

Edenberg H, Waqar M, Huberman J . DNA synthesis by partially purified replicating simian virus 40 chromosomes. Nucleic Acids Res. 1977; 4(9):3083-95. PMC: 342636. DOI: 10.1093/nar/4.9.3083. View

Edenberg H, Anderson S, DePamphilis M . Involvement of DNA polymerase alpha in simian virus 40 DNA replication. J Biol Chem. 1978; 253(9):3273-80. View

Otto B, Fanning E . DNA polymerase alpha is associated with replicating SV40 nucleoprotein complexes. Nucleic Acids Res. 1978; 5(5):1715-28. PMC: 342115. DOI: 10.1093/nar/5.5.1715. View

Waqar M, Evans M, Huberman J . Effect of 2',3'-dideoxythymidine-5'-triphosphate on HeLa cell in vitro DNA synthesis: evidence that DNA polymerase alpha is the only polymerase required for cellular DNA replication. Nucleic Acids Res. 1978; 5(6):1933-46. PMC: 342135. DOI: 10.1093/nar/5.6.1933. View

Su R, DePamphilis M . Simian virus 40 DNA replication in isolated replicating viral chromosomes. J Virol. 1978; 28(1):53-65. PMC: 354247. DOI: 10.1128/JVI.28.1.53-65.1978. View

Krokan H, Schaffer P, DePamphilis M . Involvement of eucaryotic deoxyribonucleic acid polymerases alpha and gamma in the replication of cellular and viral deoxyribonucleic acid. Biochemistry. 1979; 18(20):4431-43. DOI: 10.1021/bi00587a025. View

Tsubota Y, Waqar M, Burke J, Milavetz B, Evans M, Kowalski D . Association of enzymes with replicating and nonreplicating simian virus 40 chromosomes. Cold Spring Harb Symp Quant Biol. 1979; 43 Pt 2:693-704. DOI: 10.1101/sqb.1979.043.01.077. View

10.

Otto B, Fanning E, Richter A . DNA polymerases and a single-strand-specific DNA-binding protein associated with simian virus 40 nucleoprotein complexes. Cold Spring Harb Symp Quant Biol. 1979; 43 Pt 2:705-8. DOI: 10.1101/sqb.1979.043.01.078. View

11.

Chen Y, Bohn E, Planck S, Wilson S . Mouse DNA polymerase alpha. Subunit structure and identification of a species with associated exonuclease. J Biol Chem. 1979; 254(22):11678-87. View

12.

Waldeck W, Spaeren U, Mastromei G, Eliasson R, REICHARD P . Replication of polyoma DNA in nuclear extracts and nucleoprotein complexes. J Mol Biol. 1979; 135(3):675-89. DOI: 10.1016/0022-2836(79)90171-2. View

13.

DePamphilis M, Wassarman P . Replication of eukaryotic chromosomes: a close-up of the replication fork. Annu Rev Biochem. 1980; 49:627-66. DOI: 10.1146/annurev.bi.49.070180.003211. View

14.

Sundin O, Varshavsky A . Terminal stages of SV40 DNA replication proceed via multiply intertwined catenated dimers. Cell. 1980; 21(1):103-14. DOI: 10.1016/0092-8674(80)90118-x. View

15.

Richter A, SCHEU R, Otto B . Replication of simian virus 40 chromatin in vitro depends on the amount of DNA polymerase alpha associated with replicating chromatin. Eur J Biochem. 1980; 109(1):67-73. DOI: 10.1111/j.1432-1033.1980.tb04768.x. View

16.

Segawa M, Sugano S, Yamaguchi N . Association of simian virus 40 T antigen with replicating nucleoprotein complexes of simian virus 40. J Virol. 1980; 35(2):320-30. PMC: 288816. DOI: 10.1128/JVI.35.2.320-330.1980. View

17.

Huberman J . New views of the biochemistry of eucaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase alpha. Cell. 1981; 23(3):647-8. DOI: 10.1016/0092-8674(81)90426-8. View

18.

Gourlie B, Pigiet V, Breaux C, Krauss M, King C, Benbow R . Polyoma virus minichromosomes: associated enzyme activities. J Virol. 1981; 38(3):826-32. PMC: 171219. DOI: 10.1128/JVI.38.3.826-832.1981. View

19.

Lee M, Tan C, Downey K, So A . Structural and functional properties of calf thymus DNA polymerase delta. Prog Nucleic Acid Res Mol Biol. 1981; 26:83-96. DOI: 10.1016/s0079-6603(08)60396-7. View

20.

Sundin O, Varshavsky A . Arrest of segregation leads to accumulation of highly intertwined catenated dimers: dissection of the final stages of SV40 DNA replication. Cell. 1981; 25(3):659-69. DOI: 10.1016/0092-8674(81)90173-2. View