Genomic Environment of Variant Surface Antigen Genes of Trypanosoma Equiperdum

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1983 Jul 1

PMID 6308614

Citations 17

Authors

A Raibaud

C Gaillard

S Longacre

U Hibner

G Buck

G Bernardi

H Eisen

Affiliations

Soon will be listed here.

Abstract

Expression of variant antigen genes in Trypanosoma equiperdum is accompanied by the duplication of a silent basic copy gene and the transposition of the copy to an expression site elsewhere in the genome. We have analyzed the genomic locations of both the basic and expression-linked copies of the T. equiperdum gene for variable surface glycoprotein VSG-1. Both copies are situated proximal to termini in both extracted DNA and in chromatin. The regions between the VSG-1 genes and the termini have a very high buoyant density in CsCl and contain an unidentified nucleoside that replaces deoxycytidine.

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Characterization of the genes specifying two metacyclic variable antigen types in Trypanosoma brucei rhodesiense.

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References

Wells R, Blair J . Studies on polynucleotides. LXXI. Sedimentation and buoyant density studies of some DNA-like polymers with repeating nucleotide sequences. J Mol Biol. 1967; 27(2):273-88. DOI: 10.1016/0022-2836(67)90020-4. View

Corneo G, Ginelli E, Soave C, Bernardi G . Isolation and characterization of mouse and guinea pig satellite deoxyribonucleic acids. Biochemistry. 1968; 7(12):4373-9. DOI: 10.1021/bi00852a033. View

Thiery J, Ehrlich S, Devillers-Thiery A, Bernardi G . Specificity of spleen acid DNAase. Eur J Biochem. 1973; 38(3):434-42. DOI: 10.1111/j.1432-1033.1973.tb03077.x. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Blattner F, Williams B, Blechl A, Faber H, Furlong L, Grunwald D . Charon phages: safer derivatives of bacteriophage lambda for DNA cloning. Science. 1977; 196(4286):161-9. DOI: 10.1126/science.847462. View

Capbern A, Giroud C, Baltz T, Mattern P . [Trypanosoma equiperdum: antigenic variations in experimental trypanosomiasis of rabbits]. Exp Parasitol. 1977; 42(1):6-13. DOI: 10.1016/0014-4894(77)90055-8. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Blackburn E, Gall J . A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena. J Mol Biol. 1978; 120(1):33-53. DOI: 10.1016/0022-2836(78)90294-2. View

Williams R, Young J, Majiwa P . Genomic rearrangements correlated with antigenic variation in Trypanosoma brucei. Nature. 1979; 282(5741):847-9. DOI: 10.1038/282847a0. View

10.

Hoeijmakers J, Frasch A, Bernards A, Borst P, Cross G . Novel expression-linked copies of the genes for variant surface antigens in trypanosomes. Nature. 1980; 284(5751):78-80. DOI: 10.1038/284078a0. View

11.

Riou G, Baltz T, Gabillot M, PAUTRIZEL R . Absence of kinetoplast DNA in a late antigenic variant of Trypanosoma equiperdum. Mol Biochem Parasitol. 1980; 1(2):97-105. DOI: 10.1016/0166-6851(80)90004-3. View

12.

Pays E, Lheureux M, Steinert M . The expression-linked copy of a surface antigen gene in Trypanosoma is probably the one transcribed. Nature. 1981; 292(5820):265-7. DOI: 10.1038/292265a0. View

13.

Pays E, VAN MEIRVENNE N, Le Ray D, Steinert M . Gene duplication and transposition linked to antigenic variation in Trypanosoma brucei. Proc Natl Acad Sci U S A. 1981; 78(5):2673-7. PMC: 319419. DOI: 10.1073/pnas.78.5.2673. View

14.

Williams R, Young J, Majiwa P, Doyle J, Shapiro S . Contextural genomic rearrangements of variable-antigen genes in Trypanosoma brucei. Cold Spring Harb Symp Quant Biol. 1981; 45 Pt 2:945-9. DOI: 10.1101/sqb.1981.045.01.111. View

15.

Pays E, Lheureux M, Steinert M . Structure and expression of a Trypanosoma brucei gambiense variant specific antigen gene. Nucleic Acids Res. 1982; 10(10):3149-63. PMC: 320697. DOI: 10.1093/nar/10.10.3149. View

16.

Williams R, Young J, Majiwa P . Genomic environment of T. brucei VSG genes: presence of a minichromosome. Nature. 1982; 299(5882):417-21. DOI: 10.1038/299417a0. View

17.

de Lange T, Borst P . Genomic environment of the expression-linked extra copies of genes for surface antigens of Trypanosoma brucei resembles the end of a chromosome. Nature. 1982; 299(5882):451-3. DOI: 10.1038/299451a0. View

18.

Katinka M, Yaniv M . Deletions of N-terminal sequences of polyoma virus T-antigens reduce but do not abolish transformation of rat fibroblasts. Mol Cell Biol. 1982; 2(10):1238-46. PMC: 369923. DOI: 10.1128/mcb.2.10.1238-1246.1982. View

19.

Longacre S, Hibner U, Raibaud A, Eisen H, Baltz T, Giroud C . DNA rearrangements and antigenic variation in Trypanosoma equiperdum: multiple expression-linked sites in independent isolates of trypanosomes expressing the same antigen. Mol Cell Biol. 1983; 3(3):399-409. PMC: 368548. DOI: 10.1128/mcb.3.3.399-409.1983. View

20.

Longacre S, Raibaud A, Hibner U, Buck G, Eisen H, Baltz T . DNA rearrangements and antigenic variation in Trypanosoma equiperdum: expression-independent DNA rearrangements in the basic copy of a variant surface glycoprotein gene. Mol Cell Biol. 1983; 3(3):410-4. PMC: 368549. DOI: 10.1128/mcb.3.3.410-414.1983. View