A Radioimmunoassay for 1,25-dihydroxycholecalciferol

Overview

Journal Clin Sci Mol Med

Specialties Biochemistry
General Medicine
Genetics

Date 1978 Mar 1

PMID 630809

Citations 9

Authors

T L Clemens

G N Hendy

R F Graham

E G Baggiolini

M R Uskokovic

J L ORIORDAN

Affiliations

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Abstract

1. 1alpha,25-Dihydroxy-25-hemisuccinate cholecalciferol has been synthesized and conjugated to bovine serum albumin. 2. This conjugate is immunogenic; when injected into rabbits antibodies of high affinity for 1alpha,25-dihydroxycholecalciferol were obtained. 3. Vitamin D metabolites lacking the 1alpha-hydroxy group were of lower cross-reactivity with the antibodies. 4. By using these antibodies and 1alpha,25-[23,24-3H]dihydroxycholecalciferol as tracer a sensitive radioimmunoassay has been developed capable of detecting 20 pg of 1alpha,25-dihydroxycholecalciferol.

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