Polar Redistribution of Na+K+ATPase in Aggregating MDCK Cells

The distribution of Na+K+ATPase was examined immunoelectronmicroscopically along the plasma membranes of aggregating MDCK cells grown in tissue culture. Na+K+ATPase was localized with the use of affinity-purified antibodies employing the double-antibody immunoperoxidase technique. Na+K+ATPase-like immunoreactivity was uniformly distributed along the plasmalemma of cells grown in suspension. Once attached to the substratum, individual MDCK cells exhibited Na+K+ATPase immunoreactivity limited to the microvilli-laden mucosal surface. This exclusive mucosal distribution of Na+K+ATPase disappeared and a basolateral immunoreactivity became apparent once intercellular contacts and the formation of tight junctions occurred between neighboring cells. Thus, both cellular attachment to the substratum, and tight junction formation between aggregating MDCK cells are essential to the genesis of Na+K+ATPase polar distribution. These two events, however, appear to induce opposing distribution patterns for Na+K+ATPase.