RNA Splice Site Selection: Evidence for a 5' Leads to 3' Scanning Model

Overview

Journal Science

Specialty Science

Date 1983 Jun 24

PMID 6304877

Citations 34

Authors

K M Lang

R A Spritz

Affiliations

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Abstract

Human G gamma-globin genes containing tandem duplications of the donor (5') or acceptor (3') RNA splice sites of the second intervening sequence were constructed in order to ascertain the directionality of RNA splice site selection. These genes were introduced into cultured monkey cells, and their transcripts were analyzed. Transcripts of these duplication variants were spliced only at the proximal copy of the duplicated splice sites. These data are consistent with a 5' leads to 3' model of splice site selection.

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