Restriction Endonuclease BglI As a Tool for in Vitro Reconstruction and Recombination of Plasmid and Bacteriophage Genomes

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1983 Jan 1

PMID 6304467

Citations 5

Authors

K J Burger

R Schinzel

Affiliations

Soon will be listed here.

Abstract

The restriction endonuclease BglI produces different individual fragment ends from different cut sites. This property has allowed us to reconstruct efficiently several commonly used plasmid and bacteriophage genomes and a number of recombinant plasmids containing up to seven BglI restriction sites from their constituent BglI fragments. It is demonstrated that in vitro reconstitution from BglI fragments can be used to create, in a simple way, recombinant DNA molecules by recombining in vitro BglI fragments from different mutated or otherwise related genomes. Further applications of the method are discussed.

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