Identification of Specific Restriction Fragments Associated with a Membrane Subparticle from Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1983 Jun 1

PMID 6304013

Citations 6

Authors

M G Sargent

M F Bennett

I D Burdett

Affiliations

Soon will be listed here.

Abstract

When lysates of Bacillus subtilis were treated with restriction endonucleases EcoRI or HindIII, almost all of the DNA was released from the major plasma membrane fraction that was sedimentable at low speed. However, a very small part of the released DNA, when centrifuged at high speed, appeared to be bound to small membrane fragments. On agarose gels, this material, prepared with either enzyme, contained only a small number of restriction fragments, and the DNA in the sample hybridized with 11 to 12 EcoRI or HindIII fragments of chromosomal DNA. This DNA was used after nick-translation to screen Charon 4A clone banks for phages containing membrane-bound fragments. One of these was studied in detail. Only a part (about 5 kilobases) of the region present in this clone is important in binding the DNA to the membrane subparticle.

Citing Articles

Membrane particles from Escherichia coli and Bacillus subtilis, containing penicillin-binding proteins and enriched for chromosomal-origin DNA.

Bone E, Todd J, Ellar D, Sargent M, Wyke A J Bacteriol. 1985; 164(1):192-200.

PMID: 3930465 PMC: 214229. DOI: 10.1128/jb.164.1.192-200.1985.

Amplification of a major membrane-bound DNA sequence of Bacillus subtilis.

Sargent M, Bennett M J Bacteriol. 1985; 161(2):589-95.

PMID: 3918019 PMC: 214923. DOI: 10.1128/jb.161.2.589-595.1985.

Growth kinetics of individual Bacillus subtilis cells and correlation with nucleoid extension.

Burdett I, Kirkwood T, Whalley J J Bacteriol. 1986; 167(1):219-30.

PMID: 3087953 PMC: 212864. DOI: 10.1128/jb.167.1.219-230.1986.

Identification of a specific membrane-particle-associated DNA sequence in Bacillus subtilis.

Sargent M, Bennett M J Bacteriol. 1986; 166(1):38-43.

PMID: 3007440 PMC: 214553. DOI: 10.1128/jb.166.1.38-43.1986.

In vitro type II binding of chromosomal DNA to membrane in Bacillus subtilis.

Sato Y, McCollum M, McKenzie T, Laffan J, Zuberi A, Sueoka N J Bacteriol. 1991; 173(23):7732-5.

PMID: 1938973 PMC: 212549. DOI: 10.1128/jb.173.23.7732-7735.1991.

References

Rosenberg B, CAVALIERI L . Shear sensitivity of the E. coli genome: multiple membrane attachment points of the E. coli DNA. Cold Spring Harb Symp Quant Biol. 1968; 33:65-72. DOI: 10.1101/sqb.1968.033.01.012. View

Kourilsky P, Leidner J, TREMBLAY G . DNA-DNA hybridization on filters at low temperature in the presence of formamide or urea. Biochimie. 1971; 53(10):1111-4. DOI: 10.1016/s0300-9084(71)80201-8. View

Ivarie R, PENE J . Association of many regions of the Bacillus subtilis chromosome with the cell membrane. J Bacteriol. 1973; 114(2):571-6. PMC: 251811. DOI: 10.1128/jb.114.2.571-576.1973. View

Sargent M . Membrane synthesis in synchronous cultures of Bacillus subtilis 168. J Bacteriol. 1973; 116(1):397-409. PMC: 246436. DOI: 10.1128/jb.116.1.397-409.1973. View

Dworsky P, Schaechter M . Effect of rifampin on the structure and membrane attachment of the nucleoid of Escherichia coli. J Bacteriol. 1973; 116(3):1364-74. PMC: 246496. DOI: 10.1128/jb.116.3.1364-1374.1973. View

Leibowitz P, Schaechter M . The attachment of the bacterial chromosome to the cell membrane. Int Rev Cytol. 1975; 41:1-28. DOI: 10.1016/s0074-7696(08)60964-x. View

Parker D, Glaser D . Effect of growth conditions on DNA-membrane attachment in Escherichia coli. Proc Natl Acad Sci U S A. 1975; 72(6):2446-50. PMC: 432776. DOI: 10.1073/pnas.72.6.2446. View

Portalier R, WORCEL A . Association of the folded chromosome with the cell envelope of E. coli: characterization of the proteins at the DNA-membrane attachment site. Cell. 1976; 8(2):245-55. DOI: 10.1016/0092-8674(76)90008-8. View

Yamaguchi K, Yoshikawa H . Chromosome--Membrane association in Bacillus subtilis. III. Isolation and characterization of a DNA-protein complex carrying replication origin markers. J Mol Biol. 1977; 110(2):219-53. DOI: 10.1016/s0022-2836(77)80070-3. View

10.

Benton W, Davis R . Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977; 196(4286):180-2. DOI: 10.1126/science.322279. View

11.

Abe M, Brown C, Hendrickson W, Boyd D, Clifford P, Cote R . Release of Escherichia coli DNA from membrane complexes by single-strand endonucleases. Proc Natl Acad Sci U S A. 1977; 74(7):2756-60. PMC: 431278. DOI: 10.1073/pnas.74.7.2756. View

12.

Jeffreys A, Flavell R . A physical map of the DNA regions flanking the rabbit beta-globin gene. Cell. 1977; 12(2):429-39. DOI: 10.1016/0092-8674(77)90119-2. View

13.

Drlica K, Burgi E, WORCEL A . Association of the folded chromosome with the cell envelope of Escherichia coli: nature of the membrane-associated DNA. J Bacteriol. 1978; 134(3):1108-16. PMC: 222361. DOI: 10.1128/jb.134.3.1108-1116.1978. View

14.

Nicolaidis A, Holland I . Evidence for the specific association of the chromosomal origin with outer membrane fractions isolated from Escherichia coli. J Bacteriol. 1978; 135(1):178-89. PMC: 224803. DOI: 10.1128/jb.135.1.178-189.1978. View

15.

Valenzuela M, Inman R . Restriction enzyme cleavage of DNA resulting from gently lysed Escherichia coli. Mol Gen Genet. 1978; 166(3):245-9. DOI: 10.1007/BF00267615. View

16.

Wahl G, Stern M, Stark G . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979; 76(8):3683-7. PMC: 383897. DOI: 10.1073/pnas.76.8.3683. View

17.

Wolf-Watz H, Masters M . Deoxyribonucleic acid and outer membrane: strains diploid for the oriC region show elevated levels of deoxyribonucleic acid-binding protein and evidence for specific binding of the oriC region to outer membrane. J Bacteriol. 1979; 140(1):50-8. PMC: 216778. DOI: 10.1128/jb.140.1.50-58.1979. View

18.

Nagai K, Hendrickson W, Balakrishnan R, Yamaki H, Boyd D, Schaechter M . Isolation of a replication origin complex from Escherichia coli. Proc Natl Acad Sci U S A. 1980; 77(1):262-6. PMC: 348249. DOI: 10.1073/pnas.77.1.262. View

19.

DE Wet J, Daniels D, Schroeder J, Williams B, Moore D, Blattner F . Restriction maps for twenty-one Charon vector phages. J Virol. 1980; 33(1):401-10. PMC: 288556. DOI: 10.1128/JVI.33.1.401-410.1980. View

20.

Southern E . Gel electrophoresis of restriction fragments. Methods Enzymol. 1979; 68:152-76. DOI: 10.1016/0076-6879(79)68011-4. View