Pyridoxal 5'-phosphate: a Possible Physiological Substrate for Alkaline Phosphatase in Human Neutrophils

Overview

Journal Histochem J

Specialty Biochemistry

Date 1983 Mar 1

PMID 6303989

Citations 7

Authors

P D Wilson

G P Smith

T J Peters

Affiliations

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Abstract

The intracellular localization of pyridoxal phosphatase activity was demonstrated in human neutrophils by electron microscope cytochemistry. Under alkaline conditions, an enzyme active against pyridoxal phosphate was localized to a cytoplasmic granule population, the phosphasome. These granules have previously been shown by electron microscope cytochemical techniques and by subcellular fractionation to be rich in alkaline phosphatase. Under acidic conditions, a phosphatase activity against pyridoxal phosphate was localized to intracellular multilamellar bodies resembling secondary lysosomes. These were quite distinct from the primary, secondary and phosphasome granules and this unique localization corresponds to that previously demonstrated (tertiary granules) by subcellular fractionation studies of these cells. The similarity in the enzyme reaction requirements of alkaline pyridoxal phosphatase and alkaline phosphatase, and their localization to the same subcellular organelle, suggests that pyridoxal phosphate may be a physiological substrate for human neutrophil alkaline phosphatase.

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References

Smith G, Peters T . Studies on the activities, kinetic properties and subcellular localisation of cyclic AMP phosphodiesterase in human neutrophil leukocytes. Clin Chim Acta. 1980; 103(2):193-201. DOI: 10.1016/0009-8981(80)90213-2. View

HINES J . Effect of pyridoxine plus chronic phlebotomy on the function and morphology of bone marrow and liver in pyridoxine-responsive sideroblastic anemia. Semin Hematol. 1976; 13(2):133-40. View

French S, Castagna J . Some effects of chronic ethanol feeding on vitamin B 6 deficiency in the rat. Lab Invest. 1967; 16(4):526-31. View

Bishayee S, BACHHAWAT B . Subcellular distribution, age dependent variation and species differences of brain pyridoxal phosphate phosphatase. Neurobiology. 1972; 2(1):12-20. View

Rinehart J, GREENBERG L . Arteriosclerotic lesions in pyridoxine-deficient monkeys. Am J Pathol. 1949; 25(3):481-91. PMC: 1942913. View

Wilson P, Rustin G, Peters T . The ultrastructural localization of human neutrophil alkaline phosphatase in normal individuals during pregnancy and in patients with chronic granulocytic leukaemia. Histochem J. 1981; 13(1):31-43. DOI: 10.1007/BF01005837. View

Smith G, Peters T . Subcellular localization and properties of adenosine diphosphatase activity in human polymorphonuclear leukocytes. Biochim Biophys Acta. 1981; 673(3):234-42. View

Smith G, Smith G, Peters T . A highly sensitive fluorimetric assay for pyridoxal phosphate phosphatase. Clin Chim Acta. 1981; 114(2-3):257-62. DOI: 10.1016/0009-8981(81)90398-3. View

Rustin G, Wilson P, Peters T . Studies on the subcellular localization of human neutrophil alkaline phosphatase. J Cell Sci. 1979; 36:401-12. DOI: 10.1242/jcs.36.1.401. View

10.

Smith G, Peters T . Subcellular localization and properties of pyridoxal phosphate phosphatases of human polymorphonuclear leukocytes and their relationship to acid and alkaline phosphatase. Biochim Biophys Acta. 1981; 661(2):287-94. DOI: 10.1016/0005-2744(81)90017-6. View

11.

Lumeng L, Cleary R, Wagner R . Adequacy of vitamin B6 supplementation during pregnancy: a prospective study. Am J Clin Nutr. 1976; 29(12):1376-83. DOI: 10.1093/ajcn/29.12.1376. View

12.

WACHSTEIN M, Kellner J, Ortiz J . Pyridoxal phosphate in plasma and leukocytes in patients with leukemia and other diseases. Proc Soc Exp Biol Med. 1960; 105:563-6. DOI: 10.3181/00379727-105-26177. View

13.

Ebadi M, Govitrapong P . Microassay and properties of pyridoxal phosphate phosphatase in rat pineal gland. Int J Biochem. 1979; 10(8):705-11. DOI: 10.1016/0020-711x(79)90216-7. View

14.

Lumeng L, Li T . Characterization of the pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate hydrolase activity in rat liver. Identity with alkaline phosphatase. J Biol Chem. 1975; 250(20):8126-31. View

15.

Smith G, Peters T . Subcellular localization and properties of adenosine triphosphatase in human polymorphonuclear leucocytes. Eur J Clin Invest. 1980; 10(6):475-80. DOI: 10.1111/j.1365-2362.1980.tb02088.x. View

16.

Smith G, Peters T . Subcellular localisation and properties of histone phosphate phosphatase in human polymorphonuclear leukocytes: alterations in pregnancy and chronic granulocytic leukaemia and relationship to alkaline phosphatase. Clin Chim Acta. 1981; 111(1):17-25. DOI: 10.1016/0009-8981(81)90415-0. View

17.

Ellis J, Folkers K, Levy M, Takemura K, Shizukuishi S, Ulrich R . Therapy with vitamin B6 with and without surgery for treatment of patients having the idiopathic carpal tunnel syndrome. Res Commun Chem Pathol Pharmacol. 1981; 33(2):331-44. View

18.

Raja K, Smith G, Peters T . Subcellular localization and properties of alkaline inorganic pyrophosphatase in human polymorphonuclear leucocytes. Clin Chim Acta. 1981; 117(1):33-41. DOI: 10.1016/0009-8981(81)90007-3. View

19.

Rustin G, Peters T . Studies on the subcellular organelles of neutrophils in chronic granulocytic leukaemia with special reference to alkaline phosphatase. Br J Haematol. 1979; 41(4):533-43. DOI: 10.1111/j.1365-2141.1979.tb05891.x. View