Side Effects of Phosphorylated Acetylcholinesterase Reactivators on Neuronal Membrane and Synaptic Transmission

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1983 Jan 1

PMID 6300753

Citations 3

Authors

P Fossier

L Tauc

G Baux

Affiliations

Soon will be listed here.

Abstract

The side effects of four phosphorylated cholinesterase reactivators (oximes): contrathion, TMB4, toxogonine and 1574 SEBC on membrane properties and synaptic transmission of Aplysia central neurons were investigated. Applied in the bath at 10(-3) mol X 1(-1) to 10(-2) mol X 1(-1) concentrations, all these oximes had a depressive action on cholinergic transmission exerting a curare-like effect on the postsynaptic receptors. In addition, Toxogonin and TMB4 affected the presynaptic voltage dependent sodium conductance. None of these oximes interfered with the voltage dependent potassium or calcium conductances. The oximes had a transient facilitatory action on amplitude of the response to ionophoretically applied acetylcholine (ACh) on H-type ACh receptors, but not on cells with D-type ACh receptors. The K+ dependent response to ACh injection on pleural ganglion cells was selectively blocked by 5 X 10(-6) mol X 1(-1) contrathion. All oximes at 10(-2) mol X 1(-1) to 10(-3) mol X 1(-1) similarly depressed serotonin receptors in buccal ganglion cells. All the effects of oximes were reversible by washing. It was concluded that oximes can act as 1) inhibitors of Na+ conductance, 2) antagonists for various synaptic receptors, 3) reversible inhibitors of acetylcholinesterase.

Citing Articles

Effects of obidoxime chloride on native and sarin-poisoned frog neuromuscular junctions.

Caratsch C, Waser P Pflugers Arch. 1984; 401(1):84-90.

PMID: 6382149 DOI: 10.1007/BF00581537.

Direct and indirect effects of an organophosphorus acetylcholinesterase inhibitor and of an oxime on a neuro-neuronal synapse.

Fossier P, Baux G, Tauc L Pflugers Arch. 1983; 396(1):15-22.

PMID: 6300751 DOI: 10.1007/BF00584692.

Both presynaptic nicotinic-like and muscarinic-like autoreceptors regulate acetylcholine release at an identified neuro-neuronal synapse of Aplysia.

Fossier P, Poulain B, Baux G, Tauc L Pflugers Arch. 1988; 411(4):345-52.

PMID: 2899868 DOI: 10.1007/BF00587712.

Receptor-mediated presynaptic facilitation of quantal release of acetylcholine induced by pralidoxime in Aplysia.

Fossier P, Baux G, Poulain B, Tauc L Cell Mol Neurobiol. 1990; 10(3):383-404.

PMID: 2253262 PMC: 11567356. DOI: 10.1007/BF00711182.

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