Transport and Cleavage of Bacterial Pre-beta-lactamase by Mammalian Microsomes

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1983 Apr 1

PMID 6300144

Citations 5

Authors

H M Kronenberg

B J Fennick

T J Vasicek

Affiliations

Soon will be listed here.

Abstract

Eucaryotic endoplasmic reticulum and bacterial inner cell membranes use strikingly similar mechanisms to transport proteins from one cellular compartment to another. Using a mammalian linked transcription-translation system, we show here that canine pancreatic microsomes accurately cleave off the signal sequence of beta-lactamase, a secreted bacterial protein. We suggest that the apparent differences between bacterial and eukaryotic protein transport may involve only minor modulations of a profoundly conserved mechanism.

Citing Articles

In vitro translocation of bacterial proteins across the plasma membrane of Escherichia coli.

Muller M, Blobel G Proc Natl Acad Sci U S A. 1984; 81(23):7421-5.

PMID: 6390437 PMC: 392158. DOI: 10.1073/pnas.81.23.7421.

Secretion of human parathyroid hormone from rat pituitary cells infected with a recombinant retrovirus encoding preproparathyroid hormone.

Hellerman J, Cone R, Potts Jr J, Rich A, Mulligan R, Kronenberg H Proc Natl Acad Sci U S A. 1984; 81(17):5340-4.

PMID: 6089196 PMC: 391699. DOI: 10.1073/pnas.81.17.5340.

Soluble factors stimulating secretory protein translocation in bacteria and yeast can substitute for each other.

Fecycz I, Blobel G Proc Natl Acad Sci U S A. 1987; 84(11):3723-7.

PMID: 3295868 PMC: 304948. DOI: 10.1073/pnas.84.11.3723.

The NH2 terminus of preproinsulin directs the translocation and glycosylation of a bacterial cytoplasmic protein by mammalian microsomal membranes.

Eskridge E, Shields D J Cell Biol. 1986; 103(6 Pt 1):2263-72.

PMID: 3023397 PMC: 2114610. DOI: 10.1083/jcb.103.6.2263.

The reaction specificities of the thylakoidal processing peptidase and Escherichia coli leader peptidase are identical.

Halpin C, Elderfield P, James H, Zimmermann R, Dunbar B, Robinson C EMBO J. 1989; 8(12):3917-21.

PMID: 2684650 PMC: 402083. DOI: 10.1002/j.1460-2075.1989.tb08572.x.

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