Effects of Tn5 Insertion in the IncD Region on Mini-F Maintenance and Polypeptide Synthesis

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1982 Jan 1

PMID 6296635

Citations 6

Authors

S H Phua

P L Bergquist

H E Lane

Affiliations

Soon will be listed here.

Abstract

The gene coding for the 33,000 dalton protein (B protein) of the mini-F plasmid has been mapped by minicell polypeptide analysis to 47.3-48.2 Fkb. Transcription of the gene is initiated near 47.3 Fkb. Gene B mutants overproduce the 42,000 dalton protein (A protein) of mini-F. Insertions of Tn5 in the B gene and in the incD region cause instability of plasmid inheritance. Mini-F plasmids deleted for part of gene B are not maintained in the absence of the incD region. B protein and the incD region appear to interact in promoting mini-F maintenance.

Citing Articles

IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group.

Gardner R, Malcolm L, Bergquist P, Lane H Mol Gen Genet. 1982; 188(2):345-52.

PMID: 6759876 DOI: 10.1007/BF00332699.

Twelve 43-base-pair repeats map in a cis-acting region essential for partition of plasmid mini-F.

Helsberg M, Eichenlaub R J Bacteriol. 1986; 165(3):1043-5.

PMID: 3949712 PMC: 214538. DOI: 10.1128/jb.165.3.1043-1045.1986.

Chromosomal genes essential for stable maintenance of the mini-F plasmid in Escherichia coli.

Niki H, Ichinose C, Ogura T, Mori H, Morita M, Hasegawa M J Bacteriol. 1988; 170(11):5272-8.

PMID: 3053654 PMC: 211601. DOI: 10.1128/jb.170.11.5272-5278.1988.

Analysis of the F plasmid centromere.

Lane D, Rothenbuehler R, Merrillat A, Aiken C Mol Gen Genet. 1987; 207(2-3):406-12.

PMID: 3039298 DOI: 10.1007/BF00331608.

D protein of miniF plasmid acts as a repressor of transcription and as a site-specific resolvase.

Lane D, de Feyter R, Kennedy M, Phua S, Semon D Nucleic Acids Res. 1986; 14(24):9713-28.

PMID: 3027661 PMC: 341330.

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