Recombination During Gene Transfer into Mouse Cells Can Restore the Function of Deleted Genes

Overview

Journal Science

Specialty Science

Date 1983 Jan 14

PMID 6294829

Citations 68

Authors

J Small

G Scangos

Affiliations

Soon will be listed here.

Abstract

Two plasmids containing nonoverlapping deletions of the herpes simplex virus thymidine kinase gene were introduced into thymidine kinase-deficient mouse L cells by DNA-mediated gene transfer. Thymidine kinase-producing transformants were generated by a mixture of the two plasmids at a frequency significantly greater than that generated by either plasmid alone. Southern blot analyses demonstrated that functional thymidine kinase genes were generated by homologous recombination between the two deletion mutants.

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