The Subcellular Fractionation of the Bovine Caudate Nucleus

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1982 Sep 1

PMID 6294542

Authors

M R Gregg

S Spanner

G B Ansell

Affiliations

Soon will be listed here.

Abstract

Two synaptosomal fractions could be obtained from bovine caudate nucleus on sucrose density gradients one of which had a much greater capacity for 'high affinity' choline uptake than the other but comparable amounts of CAT and choline kinase activity. Specific binding of QNB was widely distributed among all the subcellular fractions except the mitochondrial fraction and in quantitative terms by far the greatest amount was in the microsomal fraction. Only the microsomal fraction contained measurable amounts of glycerophosphocholine phosphodiesterase.

Citing Articles

A critique on the preparation and enzymatic characterization of synaptic and nonsynaptic mitochondria from hippocampus.

Villa R, Gorini A, Lo Faro A, DellOrbo C Cell Mol Neurobiol. 1989; 9(2):247-62.

PMID: 2743381 PMC: 11567230. DOI: 10.1007/BF00713032.

References

Sorimachi M, Kataoka K . Choline uptake by nerve terminals: a sensitive and a specific marker of cholinergic innervation. Brain Res. 1974; 72(2):350-3. DOI: 10.1016/0006-8993(74)90880-4. View

Fonnum F . Choline acetyltransferase binding to and release from membranes. Biochem J. 1968; 109(3):389-98. PMC: 1186833. DOI: 10.1042/bj1090389. View

Bajgar J, Patocka J, Ornst F, Zizkovsky V, Lacina P . Subcellular distribution of acetylcholinesterase activity in human brain caudate nucleus. J Neurochem. 1971; 18(3):529-30. DOI: 10.1111/j.1471-4159.1971.tb11981.x. View

Hokfelt T, Jonsson G, Linbrink P . Electron microscopic identification of monoamine nerve ending particles in rat brain homogenates. Brain Res. 1970; 22(1):147-51. DOI: 10.1016/0006-8993(70)90412-9. View

Wheeler D . A model of high affinity choline transport in rat cortical synaptosomes. J Neurochem. 1979; 32(4):1197-213. DOI: 10.1111/j.1471-4159.1979.tb11047.x. View

Wastek G, Stern L, Johnson P, Yamamura H . Huntington's disease: regional alteration in muscarinic cholinergic receptor binding in human brain. Life Sci. 1976; 19(7):1033-9. DOI: 10.1016/0024-3205(76)90295-2. View

Gray E, Whittaker V . The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation. J Anat. 1962; 96:79-88. PMC: 1244174. View

Lehmann J, Fibiger H . Acetylcholinesterase in the substantia nigra and caudate-putamen of the rat: properties and localization in dopaminergic neurons. J Neurochem. 1978; 30(3):615-24. DOI: 10.1111/j.1471-4159.1978.tb07816.x. View

Fonnum F . Isolation of choline esters from aqueous solutions by extraction with sodium tetraphenylboron in organic solvents. Biochem J. 1969; 113(2):291-8. PMC: 1184635. DOI: 10.1042/bj1130291. View

10.

Kuhar M, DeHaven R, Yamamura H, Simon J . Further evidence for cholinergic habenulo-interpeduncular neurons: pharmacologic and functional characteristics. Brain Res. 1975; 97(2):265-75. DOI: 10.1016/0006-8993(75)90449-7. View

11.

Simon J, Atweh S, Kuhar M . Sodium-dependent high affinity choline uptake: a regulatory step in the synthesis of acetylcholine. J Neurochem. 1976; 26(5):909-22. DOI: 10.1111/j.1471-4159.1976.tb06472.x. View

12.

Hickey S, Ansell G, Mitchell K, Pearce G . Subcellular fractions of normal human substantia nigra and caudate nucleus; a study of their morphology and some enzymes including glutamate decarboxylase and choline acetyltransferase. J Neurochem. 1976; 27(4):957-62. DOI: 10.1111/j.1471-4159.1976.tb05161.x. View

13.

De Robertis E, PELLEGRINO DE IRALDI A, RODRIGUEZ DE LORES GARNAIZ G, Salganicoff L . Cholinergic and non-cholinergic nerve endings in rat brain. I. Isolation and subcellular distribution of acetylcholine and acetylcholinesterase. J Neurochem. 1962; 9:23-35. DOI: 10.1111/j.1471-4159.1962.tb07489.x. View

14.

Farrow J, OBrien R . Binding of atropine and muscarone to rat brain fractions and its relation to the acetylcholine receptor. Mol Pharmacol. 1973; 9(1):33-40. View

15.

Cotman C, Matthews D . Synaptic plasma membranes from rat brain synaptosomes: isolation and partial characterization. Biochim Biophys Acta. 1971; 249(2):380-94. DOI: 10.1016/0005-2736(71)90117-9. View

16.

Yamamura H, Snyder S . Muscarinic cholinergic binding in rat brain. Proc Natl Acad Sci U S A. 1974; 71(5):1725-9. PMC: 388311. DOI: 10.1073/pnas.71.5.1725. View

17.

Bernstein H, Weiss J, LUPPA H . Cytochemical investigations on the localization of 5'-nucleotidase in the rat hippocampus with special reference to synaptic regions. Histochemistry. 1978; 55(3):261-7. DOI: 10.1007/BF00495765. View

18.

Michell R, HAWTHORNE J . The site of diphosphoinositide synthesis in rat liver. Biochem Biophys Res Commun. 1965; 21(4):333-8. DOI: 10.1016/0006-291x(65)90198-1. View

19.

Laverty R, MICHAELSON I, SHARMAN D, Whittaker V . THE SUBCELLULAR LOCALIZATION OF DOPAMINE AND ACETYLCHOLINE IN THE DOG CAUDATE NUCLEUS. Br J Pharmacol Chemother. 1963; 21:482-90. PMC: 1703886. DOI: 10.1111/j.1476-5381.1963.tb02016.x. View

20.

Yamamura H, Snyder S . High affinity transport of choline into synaptosomes of rat brain. J Neurochem. 1973; 21(6):1355-74. DOI: 10.1111/j.1471-4159.1973.tb06022.x. View