Structural Features of the Herpes Simplex Virus Alpha Gene 4, 0, and 27 Promoter-regulatory Sequences Which Confer Alpha Regulation on Chimeric Thymidine Kinase Genes

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1982 Dec 1

PMID 6294341

Citations 167

Authors

S Mackem

B Roizman

Affiliations

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Abstract

Previous studies have shown that herpes simplex virus genes form three groups, alpha, beta, and gamma, whose expression is coordinately regulated and sequentially ordered in a cascade fashion. Chimeric genes constructed by fusion of the coding and 5' nontranslated leader sequences of the thymidine kinase (TK) gene to the sequences upstream from the site of initiation of transcription of alpha genes 4 and 27 are regulated as alpha genes and are induced in cells converted to TK+ phenotype by infection with TK- virus. In alpha gene 4 (S. Mackem and B. Roizman, Proc. Natl. Acad. Sci. U.S.A. 79:4917-4921, 1982), both the promoter and the regulatory region are separable and movable. The promoter permits expression but not induction when fused to TK in the noncoding leader region of the gene. The regulator, when fused to the promoter of an expressible but noninducible portion of the natural beta TK, renders the gene inducible as an alpha gene; it consists of multiple regulatory units acting cumulatively. In this paper, we report on the precise site of initiation of transcription of alpha gene 0 within the inverted b sequences of the L component of viral DNA. We also report the following. (i) The chimeric gene consisting of the coding and 5' nontranslated leader regions of the TK gene fused to portions of the domain of alpha gene 0 extending largely upstream from the site of initiation of transcription of alpha gene 0 was regulated in the same fashion as the alpha 4- and alpha 27-TK chimeras. The regulatory region in the alpha gene 0 is largely upstream from nucleotide - 140. (ii) The promoter-regulatory regions of alpha genes 0, 4, and 27 share TATA sequences, A + T-rich (consensus) sequences occurring in regulating regions of alpha genes 0 and 4 in more than one copy, and multiple G + C-rich inverted repeats. The relation of these sequences to the function of the promoter-regulatory regions of the alpha genes is discussed.

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References

Ejercito P, Kieff E, Roizman B . Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J Gen Virol. 1968; 2(3):357-64. DOI: 10.1099/0022-1317-2-3-357. View

Roizman B, Spear P . Preparation of herpes simplex virus of high titer. J Virol. 1968; 2(1):83-4. PMC: 375582. DOI: 10.1128/JVI.2.1.83-84.1968. View

Graham F, Van Der Eb A . A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973; 52(2):456-67. DOI: 10.1016/0042-6822(73)90341-3. View

Honess R, Roizman B . Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. J Virol. 1974; 14(1):8-19. PMC: 355471. DOI: 10.1128/JVI.14.1.8-19.1974. View

Jones P, Hayward G, Roizman B . Anatomy of herpes simplex virus DNA VII. alpha-RNA is homologous to noncontiguous sites in both the L and S components of viral DNA. J Virol. 1977; 21(1):268-76. PMC: 353812. DOI: 10.1128/JVI.21.1.268-276.1977. View

Costanzo F, Cassai E . Evidence that herpes simplex virus DNA is transcribed by cellular RNA polymerase B. J Virol. 1977; 21(3):996-1001. PMC: 515639. DOI: 10.1128/JVI.21.3.996-1001.1977. View

Morse L, Pereira L, Roizman B, Schaffer P . Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 X HSV-2 recombinants. J Virol. 1978; 26(2):389-410. PMC: 354077. DOI: 10.1128/JVI.26.2.389-410.1978. View

Fenwick M, Walker M . Suppression of the synthesis of cellular macromolecules by herpes simplex virus. J Gen Virol. 1978; 41(1):37-51. DOI: 10.1099/0022-1317-41-1-37. View

Preston V, Davison A, Marsden H, Timbury M, Wilkie N . Recombinants between herpes simplex virus types 1 and 2: analyses of genome structures and expression of immediate early polypeptides. J Virol. 1978; 28(2):499-517. PMC: 354299. DOI: 10.1128/JVI.28.2.499-517.1978. View

10.

Gannon F, OHare K, Perrin F, LePennec J, Benoist C, Cochet M . Organisation and sequences at the 5' end of a cloned complete ovalbumin gene. Nature. 1979; 278(5703):428-34. DOI: 10.1038/278428a0. View

11.

Colbere-Garapin F, Chousterman S, HORODNICEANU F, Kourilsky P, Garapin A . Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1979; 76(8):3755-9. PMC: 383912. DOI: 10.1073/pnas.76.8.3755. View

12.

Watson R, Preston C, Clements J . Separation and characterization of herpes simplex virus type 1 immediate-early mRNA's. J Virol. 1979; 31(1):42-52. PMC: 353420. DOI: 10.1128/JVI.31.1.42-52.1979. View

13.

Locker H, Frenkel N . BamI, KpnI, and SalI restriction enzyme maps of the DNAs of herpes simplex virus strains Justin and F: occurrence of heterogeneities in defined regions of the viral DNA. J Virol. 1979; 32(2):429-41. PMC: 353574. DOI: 10.1128/JVI.32.2.429-441.1979. View

14.

Benoist C, OHare K, Breathnach R, Chambon P . The ovalbumin gene-sequence of putative control regions. Nucleic Acids Res. 1980; 8(1):127-42. PMC: 327247. DOI: 10.1093/nar/8.1.127. View

15.

Sakonju S, Bogenhagen D, Brown D . A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region. Cell. 1980; 19(1):13-25. DOI: 10.1016/0092-8674(80)90384-0. View

16.

Roizman B . The organization of the herpes simplex virus genomes. Annu Rev Genet. 1979; 13:25-57. DOI: 10.1146/annurev.ge.13.120179.000325. View

17.

Engelke D, Ng S, Shastry B, Roeder R . Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes. Cell. 1980; 19(3):717-28. DOI: 10.1016/s0092-8674(80)80048-1. View

18.

Anderson K, Costa R, Holland L, Wagner E . Characterization of herpes simplex virus type 1 RNA present in the absence of de novo protein synthesis. J Virol. 1980; 34(1):9-27. PMC: 288666. DOI: 10.1128/JVI.34.1.9-27.1980. View

19.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

20.

Watson R, Clements J . A herpes simplex virus type 1 function continuously required for early and late virus RNA synthesis. Nature. 1980; 285(5763):329-30. DOI: 10.1038/285329a0. View