The Isolation and Partial Characterisation of a Cytomegalovirus from the Brown Rat, Rattus Norvegicus

Overview

Journal Arch Virol

Specialty Microbiology

Date 1982 Jan 1

PMID 6293409

Citations 13

Authors

P K Priscott

D A Tyrrell

Affiliations

Soon will be listed here.

Abstract

This report describes the isolation and partial characterisation of a cytomegalovirus (CMV) from the wild brown rat (Rattus norvegicus). The isolate was sensitive to ether and heat treatment at 56 degrees C/30 minutes, and had a characteristic herpes virus morphology. In rat embryo fibroblast cells, new virus was detectable by 18 hours after inoculation. The cytopathic effect consisted of a focal rounding of cells which developed to involve the entire monolayer. Inoculation of newborn rats produced mortality of 67-75 per cent which was reduced to 33-46 per cent by intra-peritoneal inoculation or tissue culture passage. Highest virus concentrations were found in the liver and spleen 14 days after inoculation. The virus was also pathogenic (to a lesser extent) to newborn BALB/c mice. It was related to the Osborn strain of mouse CMV by fluorescent-antibody testing but distinct by the serum-neutralisation test.

Citing Articles

Detection of DNA Viruses in Free-Ranging Rat Populations in Hungary.

Vidovszky M, Surjan A, Foldvari G, Egyed L Viruses. 2025; 16(12.

PMID: 39772254 PMC: 11680385. DOI: 10.3390/v16121948.

A simple method for rapid cloning of complete herpesvirus genomes.

Knickmann J, Staliunaite L, Puhach O, Ostermann E, Gunther T, Nichols J Cell Rep Methods. 2024; 4(2):100696.

PMID: 38266652 PMC: 10921015. DOI: 10.1016/j.crmeth.2024.100696.

Isolation and genome sequencing of cytomegaloviruses from Natal multimammate mice ().

Hansen F, Vucak M, Nichols J, Hughes J, Bane S, Camiolo S J Gen Virol. 2023; 104(8).

PMID: 37643006 PMC: 10721045. DOI: 10.1099/jgv.0.001873.

Rodent Models of Congenital Cytomegalovirus Infection.

Lisnic B, Tomac J, Cekinovic D, Jonjic S, Juranic Lisnic V Methods Mol Biol. 2021; 2244:365-401.

PMID: 33555596 DOI: 10.1007/978-1-0716-1111-1_18.

Complete Genome Sequence of Rat Cytomegalovirus Strain ALL-03 (Malaysian Strain).

Balakrishnan K, Abdullah A, Camalxaman S, Quah Y, Abba Y, Hani H Genome Announc. 2015; 3(3).

PMID: 26044413 PMC: 4457050. DOI: 10.1128/genomeA.00451-15.

References

Smith M . Propagation of salivary gland virus of the mouse in tissue cultures. Proc Soc Exp Biol Med. 1954; 86(3):435-40. DOI: 10.3181/00379727-86-21123. View

Rabson A, Edgcomb J, LEGALLAIS F, Tyrrell S . Isolation and growth of rat cytomegalovirus in vitro. Proc Soc Exp Biol Med. 1969; 131(3):923-7. DOI: 10.3181/00379727-131-34010. View

Waner J, Biano S . Antibody reactivity to human and vervet cytomegalovirus early antigen(s) in sera from patients with active cytomegalovirus infections and from asymptomatic donors. J Clin Microbiol. 1979; 9(1):134-40. PMC: 272970. DOI: 10.1128/jcm.9.1.134-140.1979. View

Kim K, Carp R . Growth of murine cytomegalovirus in various cell lines. J Virol. 1971; 7(6):720-5. PMC: 356190. DOI: 10.1128/JVI.7.6.720-725.1971. View

SYVERTON J, Larson C . Intranuclear inclusion bodies in the kidneys of wild rats. Arch Pathol (Chic). 2010; 43(6):541-52. View

Mims C, Gould J . Infection of salivary glands, kidneys, adrenals, ovaries and epithelia by murine cytomegalovirus. J Med Microbiol. 1979; 12(1):113-22. DOI: 10.1099/00222615-12-1-113. View

Berezesky I, Grimley P, Tyrrell S, Rabson A . Ultrastructure of a rat cytomegalovirus. Exp Mol Pathol. 1971; 14(3):337-49. DOI: 10.1016/0014-4800(71)90005-0. View

Mims C, Gould J . The role of macrophages in mice infected with murine cytomegalovirus. J Gen Virol. 1978; 41(1):143-53. DOI: 10.1099/0022-1317-41-1-143. View

Moon H, Sapienza V, Carp R, Kim K . Murine cytomegalovirus-induced protein synthesis. J Gen Virol. 1979; 42(1):159-69. DOI: 10.1099/0022-1317-42-1-159. View

10.

Jordan M, Shanley J, Stevens J . Immunosuppression reactivates and disseminates latent murine cytomegalovirus. J Gen Virol. 1977; 37(2):419-23. DOI: 10.1099/0022-1317-37-2-419. View

11.

Mims C . Intracerebral injections and the growth of viruses in the mouse brain. Br J Exp Pathol. 1960; 41:52-9. PMC: 2082358. View

12.

Olding L, JENSEN F, Oldstone M . Pathogenesis of of cytomegalovirus infection. I. Activation of virus from bone marrow-derived lymphocytes by in vitro allogenic reaction. J Exp Med. 1975; 141(3):561-72. PMC: 2189707. DOI: 10.1084/jem.141.3.561. View

13.

Tegtmeyer P, Ming P, Craighead J . Cytological studies on cytomegalovirus infected mouse cells. Arch Gesamte Virusforsch. 1969; 26(4):334-40. DOI: 10.1007/BF01250943. View

14.

KUTTNER A, Wang S . THE PROBLEM OF THE SIGNIFICANCE OF THE INCLUSION BODIES FOUND IN THE SALIVARY GLANDS OF INFANTS, AND THE OCCURRENCE OF INCLUSION BODIES IN THE SUBMAXILLARY GLANDS OF HAMSTERS, WHITE MICE, AND WILD RATS (PEIPING). J Exp Med. 2009; 60(6):773-91. PMC: 2132411. DOI: 10.1084/jem.60.6.773. View

15.

Brautigam A, Dutko F, Olding L, Oldstone M . Pathogenesis of murine cytomegalovirus infection: the macrophage as a permissive cell for cytomegalovirus infection, replication and latency. J Gen Virol. 1979; 44(2):349-59. DOI: 10.1099/0022-1317-44-2-349. View

16.

Cole R, KUTTNER A . A FILTERABLE VIRUS PRESENT IN THE SUBMAXILLARY GLANDS OF GUINEA PIGS. J Exp Med. 2009; 44(6):855-73. PMC: 2131220. DOI: 10.1084/jem.44.6.855. View

17.

Olding L, Kingsbury D, Oldstone M . Pathogenesis of cytomegalovirus infection. Distribution of viral products, immune complexes and autoimmunity during latent murine infection. J Gen Virol. 1976; 33(2):267-80. DOI: 10.1099/0022-1317-33-2-267. View

18.

Lussier G . Murine cytomegalovirus (MCMV). Adv Vet Sci Comp Med. 1975; 19:223-47. View

19.

LYON H, CHRISTIAN J, Miller C . Cytomegalic inclusion disease of lacrimal glands in male laboratory rats. Proc Soc Exp Biol Med. 1959; 101(1):164-6. DOI: 10.3181/00379727-101-24868. View