Rapid and Transient Localization of the Leader RNA of Vesicular Stomatitis Virus in the Nuclei of Infected Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Sep 1

PMID 6291035

Citations 28

Authors

M G Kurilla

H Piwnica-Worms

J D Keene

Affiliations

Soon will be listed here.

Abstract

The leader RNA transcript from the 3' end of the genome of vesicular stomatitis virus (VSV) has been detected in both the nucleus and cytoplasm of infected baby hamster kidney (BHK) cells. In the cytoplasm, leader RNA accumulated gradually throughout the infection to about 200 molecules per cell at 6 hr after infection. In the nucleus, however, there was a sharp and rapid increase in the concentration of leader RNA to approximately equal to 300 molecules per cell at about 2 hr after infection that decreased rapidly by 3 hr. This report presents evidence for nuclear localization of transcription products of a (-)-strand RNA virus other than influenza and supports the hypothesis that the leader RNA plays a role in the shutoff of host cell transcription.

Citing Articles

Defective Interfering Genomes and the Full-Length Viral Genome Trigger RIG-I After Infection With Vesicular Stomatitis Virus in a Replication Dependent Manner.

Linder A, Bothe V, Linder N, Schwarzlmueller P, Dahlstrom F, Bartenhagen C Front Immunol. 2021; 12:595390.

PMID: 33995343 PMC: 8119886. DOI: 10.3389/fimmu.2021.595390.

Experimental evolution of an oncolytic vesicular stomatitis virus with increased selectivity for p53-deficient cells.

Garijo R, Hernandez-Alonso P, Rivas C, Diallo J, Sanjuan R PLoS One. 2014; 9(7):e102365.

PMID: 25010337 PMC: 4092128. DOI: 10.1371/journal.pone.0102365.

Influenza A virus expresses high levels of an unusual class of small viral leader RNAs in infected cells.

Umbach J, Yen H, Poon L, Cullen B mBio. 2010; 1(4).

PMID: 20842206 PMC: 2934610. DOI: 10.1128/mBio.00204-10.

Vesicular stomatitis virus mRNA capping machinery requires specific cis-acting signals in the RNA.

Wang J, McElvain L, Whelan S J Virol. 2007; 81(20):11499-506.

PMID: 17686869 PMC: 2045530. DOI: 10.1128/JVI.01057-07.

Reviewing Chandipura: a vesiculovirus in human epidemics.

Basak S, Mondal A, Polley S, Mukhopadhyay S, Chattopadhyay D Biosci Rep. 2007; 27(4-5):275-98.

PMID: 17610154 PMC: 7087735. DOI: 10.1007/s10540-007-9054-z.

References

Weinberg R, Penman S . Small molecular weight monodisperse nuclear RNA. J Mol Biol. 1968; 38(3):289-304. DOI: 10.1016/0022-2836(68)90387-2. View

Follett E, Pringle C, Wunner W, Skehel J . Virus replication in enucleate cells: vesicular stomatitis virus and influenza virus. J Virol. 1974; 13(2):394-9. PMC: 355309. DOI: 10.1128/JVI.13.2.394-399.1974. View

Zieve G, Penman S . Small RNA species of the HeLa cell: metabolism and subcellular localization. Cell. 1976; 8(1):19-31. DOI: 10.1016/0092-8674(76)90181-1. View

Keene J, Rosenberg M, Lazzarini R . Characterization of the 3' terminus of RNA isolated from vesicular stomatitis virus and from its defective interfering particles. Proc Natl Acad Sci U S A. 1977; 74(4):1353-7. PMC: 430755. DOI: 10.1073/pnas.74.4.1353. View

Marvaldi J, Sekellick M, Marcus P . Inhibition of mouse L cell protein synthesis by ultraviolet-irradiated vesicular stomatitis virus requires viral transcription. Virology. 1978; 84(1):127-33. DOI: 10.1016/0042-6822(78)90224-6. View

Keene J, Schubert M, Lazzarini R, Rosenberg M . Nucleotide sequence homology at the 3' termini of RNA from vesicular stomatitis virus and its defective interfering particles. Proc Natl Acad Sci U S A. 1978; 75(7):3225-9. PMC: 392747. DOI: 10.1073/pnas.75.7.3225. View

Colonno R, Banerjee A . Complete nucleotide sequence of the leader RNA synthesized in vitro by vesicular stomatitis virus. Cell. 1978; 15(1):93-101. DOI: 10.1016/0092-8674(78)90085-5. View

Bouloy M, Plotch S, KRUG R . Globin mRNAs are primers for the transcription of influenza viral RNA in vitro. Proc Natl Acad Sci U S A. 1978; 75(10):4886-90. PMC: 336226. DOI: 10.1073/pnas.75.10.4886. View

Weck P, Wagner R . Vesicular stomatitis virus infection reduces the number of active DNA-dependent RNA polymerases in myeloma cells. J Biol Chem. 1979; 254(12):5430-4. View

10.

Peattie D . Direct chemical method for sequencing RNA. Proc Natl Acad Sci U S A. 1979; 76(4):1760-4. PMC: 383470. DOI: 10.1073/pnas.76.4.1760. View

11.

Weck P, Carroll A, Shattuck D, Wagner R . Use of UV irradiation to identify the genetic information of vesicular stomatitis virus responsible for shutting off cellular RNA synthesis. J Virol. 1979; 30(3):746-53. PMC: 353383. DOI: 10.1128/JVI.30.3.746-753.1979. View

12.

Simonsen C, Summers D . RNA synthesis of vesicular stomatitis virus-infected cells: in vivo regulation of replication. J Virol. 1979; 31(1):124-32. PMC: 353428. DOI: 10.1128/JVI.31.1.124-132.1979. View

13.

Leppert M, Rittenhouse L, Perrault J, Summers D, Kolakofsky D . Plus and minus strand leader RNAs in negative strand virus-infected cells. Cell. 1979; 18(3):735-47. DOI: 10.1016/0092-8674(79)90127-2. View

14.

McGowan J, Emerson S, Wagner R . The plus-strand leader RNA of VSV inhibits DNA-dependent transcription of adenovirus and SV40 genes in a soluble whole-cell extract. Cell. 1982; 28(2):325-33. DOI: 10.1016/0092-8674(82)90350-6. View

15.

Wu F . Inhibition of ribonucleic acid accumulation in mouse L cells infected with vesicular stomatitis virus requires viral ribonucleic acid transcription. Biochemistry. 1980; 19(4):804-10. DOI: 10.1021/bi00545a029. View

16.

Testa D, Chanda P, Banerjee A . Unique mode of transcription in vitro by Vesicular stomatitis virus. Cell. 1980; 21(1):267-75. DOI: 10.1016/0092-8674(80)90134-8. View

17.

Keene J, Schubert M, Lazzarini R . Intervening sequence between the leader region and the nucleopcapsid gene of vesicular stomatitis virus RNA. J Virol. 1980; 33(2):789-94. PMC: 288605. DOI: 10.1128/JVI.33.2.789-794.1980. View

18.

Gurney Jr T, Eliceiri G . Intracellular distribution of low molecular weight RNA species in HeLa cells. J Cell Biol. 1980; 87(2 Pt 1):398-403. PMC: 2110751. DOI: 10.1083/jcb.87.2.398. View

19.

HERMAN R, Schubert M, Keene J, Lazzarini R . Polycistronic vesicular stomatitis virus RNA transcripts. Proc Natl Acad Sci U S A. 1980; 77(8):4662-5. PMC: 349905. DOI: 10.1073/pnas.77.8.4662. View

20.

Lamb R, Lai C, Choppin P . Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: colinear and interrupted mRNAs code for overlapping proteins. Proc Natl Acad Sci U S A. 1981; 78(7):4170-4. PMC: 319750. DOI: 10.1073/pnas.78.7.4170. View