Noradrenergic Neurotransmission in the Brain of a Convulsive Mutant Mouse, Differences Between the Cerebral Cortex and the Brain Stem

Overview

Journal Naunyn Schmiedebergs Arch Pharmacol

Specialty Pharmacology

Date 1982 Jul 1

PMID 6289135

Citations 2

Authors

Y Maurin

S Arbilla

J Dedek

C R Lee

N Baumann

S Z Langer

Affiliations

Soon will be listed here.

Abstract

The Quaking mouse is a genetically determined model of convulsive disorders. We investigated the modulation of noradrenergic neurotransmission through alpha 2-adrenoceptors in the occipital cortex and the brain stem of this mutant. The endogenous levels of noradrenaline were similar in the cerebral cortex of the Quaking mice and their corresponding controls, while a significant increase of endogenous noradrenaline was found in the brain stem of the mutants. The rate of disappearance of noradrenaline in the cerebral cortex and the brain stem after injection of FLA 63 was identical in control and Quaking mice. The calcium-dependent electrically evoked overflow of 3H-noradrenaline from slices of occipital cortex was inhibited by clonidine and enhanced by yohimbine in Quaking as well as in normal mice. The negative feed-back mechanism mediated by presynaptic alpha 2-adrenoceptors operates to a similar extent in both strains of mice. In contrast to the occipital cortex, in the brain stem, the amount of neurotransmitter released by electrical stimulation was significantly increased in Quaking mice when compared with controls. However, in the brain stem, the negative feed-back regulation of noradrenaline release operates to a similar extent in both strains of mice. When the endogenous levels of MOPEG were determined in the brain stem, they were found to be significantly higher in the Quaking mice when compared to the controls. The results suggest that an increase in noradrenergic neurotransmission in the brain stem, rather than in the cerebral cortex, could contribute to the behavioural abnormalities exhibited by the Quaking mice.

Citing Articles

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References

Trottier S, Berger B, Chauvel P, Dedek J, Gay M . Alterations of the cortical noradrenergic system in chronic cobalt epileptogenic foci in the rat: a histofluorescent and biochemical study. Neuroscience. 1981; 6(6):1069-80. DOI: 10.1016/0306-4522(81)90071-3. View

London E, Buterbaugh G . Modification of electroshock convulsive responses and thresholds in neonatal rats after brain monoamine reduction. J Pharmacol Exp Ther. 1978; 206(1):81-90. View

Pelayo F, Dubocovich M, Langer S . Inhibition of neuronal uptake reduces the presynaptic effects of clonidine but not of alpha-methylnoradrenaline on the stimulation-evoked release of 3H-noradrenaline from rat occipital cortex slices. Eur J Pharmacol. 1980; 64(2-3):143-55. DOI: 10.1016/0014-2999(80)90037-0. View

Westerink B, Korf J . Rapid concurrent automated fluorimetric assay of noradrenaline, dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid and 3-methoxytyramine in milligram amounts of nervous tissue after isolation on Sephadex G10. J Neurochem. 1977; 29(4):697-706. DOI: 10.1111/j.1471-4159.1977.tb07788.x. View

Thierry A, Blanc G, Glowinski J . Further evidence for the heterogeneous storage of noradrenaline in central noradrenergic terminals. Naunyn Schmiedebergs Arch Pharmacol. 1973; 279(3):255-66. DOI: 10.1007/BF00500605. View

UPrichard D, Reisine T, Mason S, Fibiger H, Yamamura H . Modulation of rat brain alpha- and beta-adrenergic receptor populations by lesion of the dorsal noradrenergic bundle. Brain Res. 1980; 187(1):143-54. DOI: 10.1016/0006-8993(80)90500-4. View

Karoum F, Gillin J, Wyatt R . Mass fragmentographic determination of some acidic and alcoholic metabolites of biogenic amines in the rat brain. J Neurochem. 1975; 25(5):653-8. DOI: 10.1111/j.1471-4159.1975.tb04384.x. View

Starke K, Montel H . Influence of drugs with affinity for alpha-adrenoceptors on noradrenaline release by potassium, tyramine and dimethylphenylpiperazinium. Eur J Pharmacol. 1974; 27(3):273-80. DOI: 10.1016/0014-2999(74)90001-6. View

Chermat R, Doare L, Lachapelle F, Simon P . Effects of drugs affecting the noradrenergic system on convulsions in the quaking mouse. Naunyn Schmiedebergs Arch Pharmacol. 1981; 318(2):94-9. DOI: 10.1007/BF00508832. View

10.

Kilian M, Frey H . Central monoamines and convulsine thresholds in mice and rats. Neuropharmacology. 1973; 12(7):681-92. DOI: 10.1016/0028-3908(73)90121-4. View

11.

Braestrup C . Identification of free and conjugated 3-methoxy-4-hydroxyphenylglycol (MOPEG) in rat brain by gas chromatography and mass fragmentography. Anal Biochem. 1973; 55(2):420-31. DOI: 10.1016/0003-2697(73)90132-2. View

12.

Corcoran M, Fibiger H, McGeer E, WADA J . Potentiation of leptazol seizures by 6-hydroxydopamine. J Pharm Pharmacol. 1973; 25(6):497-9. DOI: 10.1111/j.2042-7158.1973.tb09143.x. View

13.

Arbilla S, Kamal L, Langer S . Presynaptic GABA autoreceptors on GABAergic nerve endings of the rat substantia nigra. Eur J Pharmacol. 1979; 57(2-3):211-7. DOI: 10.1016/0014-2999(79)90367-4. View

14.

Arbilla S, Langer S . Facilitation by GABA of the potassium-evoked release of 3-H-noradrenaline from the rat occipital cortex. Naunyn Schmiedebergs Arch Pharmacol. 1979; 306(2):161-8. DOI: 10.1007/BF00498986. View

15.

Mason S, Corcoran M . Catecholamines and convulsions. Brain Res. 1979; 170(3):497-507. DOI: 10.1016/0006-8993(79)90967-3. View

16.

UPrichard D, Bechtel W, Rouot B, Snyder S . Multiple apparent alpha-noradrenergic receptor binding sites in rat brain: effect of 6-hydroxydopamine. Mol Pharmacol. 1979; 16(1):47-60. View

17.

MAYNERT E, MARCZYNSKI T, Browning R . The role of the neurotransmitters in the epilepsies. Adv Neurol. 1975; 13:79-147. View

18.

Warsh J, Godse D, Cheung S, Li P . Rat brain and plasma norepinephrine glycol metabolites determined by gas chromatography-mass fragmentography. J Neurochem. 1981; 36(3):893-901. DOI: 10.1111/j.1471-4159.1981.tb01678.x. View

19.

Schlesinger K, Boggan W, Freedman D . Genetics of audiogenic seizures: II. Effects of pharmacological manipulation of brain serotonin, norepinephrine and gamma-aminobutyric acid. Life Sci. 1968; 7(7):437-47. DOI: 10.1016/0024-3205(68)90015-5. View

20.

Chermat R, Lachapelle F, Baumann N, Simon P . Anticonvulsant effect of yohimbine in quaking mice: antagonism by clonidine and prazosine. Life Sci. 1979; 25(17):1471-6. DOI: 10.1016/0024-3205(79)90372-2. View