Detection of Immunoglobulin M Antibody to Epstein-Barr Virus by Use of an Enzyme-labeled Antigen

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1982 Aug 1

PMID 6288765

Citations 13

Authors

H Schmitz

Affiliations

Soon will be listed here.

Abstract

Immunoglobulin M (IgM) antibodies to Epstein-Barr virus were detected by using microtiter plates coated with anti-mu-chain antiserum and enzyme-labeled Epstein-Barr virus antigen. Optimum conditions for labeling were determined. The addition of unlabeled control antigen to the enzyme-labeled antigen was effective in reducing background reactions. Rheumatoid factor no longer interfered. Blocking of specific IgM antibody by IgG also was no longer observed. Four different methods for the detection of acute Epstein-Barr virus infections were compared. A combination of the enzyme-labeled antigen-IgM test with the detection of antibodies to either Epstein-Barr nuclear antigen or heterophile antigen was highly sensitive and specific. By changing the solid phase, IgA antibodies to Epstein-Barr virus could be detected.

Citing Articles

Coinfection with SARS-CoV-2 and Cytomegalovirus in a Patient with Mild COVID-19.

Ura K, Goubaru Y, Motoya M, Ishii H Case Rep Infect Dis. 2023; 2023:6684783.

PMID: 37288151 PMC: 10243944. DOI: 10.1155/2023/6684783.

Primary Epstein-Barr virus infection shortly after primary Cytomegalovirus infection: a case report.

Koyama K, Anno T, Urano T, Shigemoto R, Irie S, Kawasaki F J Med Case Rep. 2021; 15(1):257.

PMID: 33941264 PMC: 8091641. DOI: 10.1186/s13256-021-02817-2.

Detection of viral antigens, particles, and early antibodies in diagnosis.

Chernesky M, Mahony J Yale J Biol Med. 1984; 57(5):757-76.

PMID: 6395513 PMC: 2589658.

Detection of antichlamydial immunoglobulin G and M antibodies by enzyme-linked immunosorbent assay.

Mahony J, Schachter J, Chernesky M J Clin Microbiol. 1983; 18(2):270-5.

PMID: 6352731 PMC: 270789. DOI: 10.1128/jcm.18.2.270-275.1983.

Detection of specific immunoglobulin M antibody to different flaviviruses by use of enzyme-labeled antigens.

Schmitz H, Emmerich P J Clin Microbiol. 1984; 19(5):664-7.

PMID: 6330165 PMC: 271151. DOI: 10.1128/jcm.19.5.664-667.1984.

References

Henie G, Henle W, Horwitz C . Antibodies to Epstein-Barr virus-associated nuclear antigen in infectious mononucleosis. J Infect Dis. 1974; 130(3):231-9. DOI: 10.1093/infdis/130.3.231. View

Nikoskelainen J, Leikola J, KLEMOLA E . IgM antibodies specific for Epstein-Barr virus in infectious mononucleosis without heterophil antibodies. Br Med J. 1974; 4(5936):72-5. PMC: 1612132. DOI: 10.1136/bmj.4.5936.72. View

Schmitz H, Doerr H, Kampa D, Vogt A . Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus. J Clin Microbiol. 1977; 5(6):629-34. PMC: 274669. DOI: 10.1128/jcm.5.6.629-634.1977. View

zur Hausen H, ONEILL F, Freese U, Hecker E . Persisting oncogenic herpesvirus induced by the tumour promotor TPA. Nature. 1978; 272(5651):373-5. DOI: 10.1038/272373a0. View

Lennette E, Henle G, Henle W, Horwitz C . Heterophil antigen in bovine sera detectable by immune adherence hemagglutination with infectious mononucleosis sera. Infect Immun. 1978; 19(3):923-7. PMC: 422280. DOI: 10.1128/iai.19.3.923-927.1978. View

Schmitz H . Improved detection of virus-specific IgM antibodies. Elimination of non-specific IgM binding. J Gen Virol. 1978; 40(2):459-63. DOI: 10.1099/0022-1317-40-2-459. View

Ertl H, Gerlich W, Koszinowski U . Detection of antibodies to Sendai virus by enzyme-linked immunosorbent assay (ELISA). J Immunol Methods. 1979; 28(1-2):163-76. DOI: 10.1016/0022-1759(79)90338-7. View

Henle G, Lennette E, Alspaugh M, Henle W . Rheumatoid factor as a cause of positive reactions in tests for Epstein-Barr virus-specific IgM antibodies. Clin Exp Immunol. 1979; 36(3):415-22. PMC: 1537758. View

Levey B, Lo T, Caldwell K, Fletcher M . Latex test for serodiagnosis of infectious mononucleosis. J Clin Microbiol. 1980; 11(3):256-62. PMC: 273374. DOI: 10.1128/jcm.11.3.256-262.1980. View

10.

Schmitz H, von Deimling U, Flehmig B . Detection of IgM antibodies to cytomegalovirus (CMV) using an enzyme-labelled antigen (ELA). J Gen Virol. 1980; 50(1):59-68. DOI: 10.1099/0022-1317-50-1-59. View

11.

de Savigny D, Voller A . The communication of ELISA data from laboratory to clinician. J Immunoassay. 1980; 1(1):105-28. DOI: 10.1080/01971528008055779. View

12.

Franco E, WALLS K, SULZER A . Reverse enzyme immunoassay for detection of specific anti-Toxoplasma immunoglobulin M antibodies. J Clin Microbiol. 1981; 13(5):859-64. PMC: 273904. DOI: 10.1128/jcm.13.5.859-864.1981. View

13.

Schmitz H, Scherer M . IgM antibodies to Epstein-Barr virus in infectious mononucleosis. Arch Gesamte Virusforsch. 1972; 37(4):332-9. DOI: 10.1007/BF01241456. View

14.

Schmitz H, Volz D, Scherer M . Acute Epstein-Barr virus infections in children. Med Microbiol Immunol. 1972; 158(1):58-63. DOI: 10.1007/BF02122009. View

15.

Bohlen P, Stein S, Dairman W, UDENFRIEND S . Fluorometric assay of proteins in the nanogram range. Arch Biochem Biophys. 1973; 155(1):213-20. DOI: 10.1016/s0003-9861(73)80023-2. View

16.

Henle W, Henle G, Horwitz C . Epstein-Barr virus specific diagnostic tests in infectious mononucleosis. Hum Pathol. 1974; 5(5):551-65. DOI: 10.1016/s0046-8177(74)80006-7. View