Erythroid Progenitors Circulating in the Blood of Adult Individuals Produce Fetal Hemoglobin in Culture

Overview

Journal Science

Specialty Science

Date 1978 Mar 24

PMID 628844

Citations 14

Authors

T Papayannopoulou

B Nakamoto

J Buckley

S Kurachi

P E Nute

G Stamatoyannopoulos

Affiliations

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Abstract

Erythroid colonies, raised from erythroid stem cells circulating in the peripheral blood of normal adult individuals, synthesize considerable amounts of fetal hemoglobin. In cultures from persons with sickling disorders, amounts of hemoglobin F that are known to inhibit sickling in vivo are produced. The results provide evidence that primitive erythroid progenitors are able to express the hemoglobin F production program and that cultures of mononuclear cells of the adult blood can be used to investigate the mechanisms involved in regulation of gamma-globin gene switching.

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A Novel High-Content Immunofluorescence Assay as a Tool to Identify at the Single Cell Level γ-Globin Inducing Compounds.

Durlak M, Fugazza C, Elangovan S, Marini M, Marongiu M, Moi P PLoS One. 2015; 10(10):e0141083.

PMID: 26509275 PMC: 4624791. DOI: 10.1371/journal.pone.0141083.

Transcriptional regulation of fetal to adult hemoglobin switching: new therapeutic opportunities.

Wilber A, Nienhuis A, Persons D Blood. 2011; 117(15):3945-53.

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Transcriptional activation of the gamma-globin gene in baboons treated with decitabine and in cultured erythroid progenitor cells involves different mechanisms.

Chin J, Singh M, Banzon V, Vaitkus K, Ibanez V, Kouznetsova T Exp Hematol. 2009; 37(10):1131-42.

PMID: 19576949 PMC: 8728742. DOI: 10.1016/j.exphem.2009.06.007.

Serum factors can modulate the developmental clock of gamma- to beta-globin gene switching in somatic cell hybrids.

Zitnik G, Li Q, Stamatoyannopoulos G, Papayannopoulou T Mol Cell Biol. 1993; 13(8):4844-51.

PMID: 8336721 PMC: 360113. DOI: 10.1128/mcb.13.8.4844-4851.1993.