Cytosine Methylation of the Sequence GATC in a Mycoplasma

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1982 Sep 1

PMID 6286600

Citations 23

Authors

K Dybvig

D Swinton

J Maniloff

S Hattman

Affiliations

Soon will be listed here.

Abstract

Mycoplasma virus L2 is subject to host-specific restriction and modification in Acholeplasma laidlawii strains JA1 and K2. We have examined the DNAs from both host cells and viruses propagated on these strains with respect to susceptibility to cleavage by restriction endonucleases and for DNA base modifications. We show that, in strain K2 and L2 virus grown on K2 cells, cytosine in the sequence GATC is methylated to 5-methylcytosine and, although strain K2 and L2 viruses grown on K2 contain N6-methyladenine in their DNA, adenine in the sequence GATC is not methylated. In contrast to K2, strain JA1 and L2 virus grown on JA1 cells contain no detectable methylated bases. It is not known which of the methylated bases in K2 is the basis for the K2 restriction-modification system operative on L2 virus.

Citing Articles

Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.

Nelson M, Raschke E, McClelland M Nucleic Acids Res. 1993; 21(13):3139-54.

PMID: 8392715 PMC: 309743. DOI: 10.1093/nar/21.13.3139.

Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.

McClelland M, Nelson M, Raschke E Nucleic Acids Res. 1994; 22(17):3640-59.

PMID: 7937074 PMC: 308336. DOI: 10.1093/nar/22.17.3640.

Polyethylene glycol-dependent transfection of Acholeplasma laidlawii with mycoplasma virus L2 DNA.

Sladek T, Maniloff J J Bacteriol. 1983; 155(2):734-41.

PMID: 6874642 PMC: 217744. DOI: 10.1128/jb.155.2.734-741.1983.

Effect of novobiocin on mycoplasma virus L2 replication.

Poddar S, Maniloff J J Virol. 1984; 49(1):283-6.

PMID: 6690717 PMC: 255456. DOI: 10.1128/JVI.49.1.283-286.1984.

5-Methylcytosine is not detectable in Saccharomyces cerevisiae DNA.

Proffitt J, Davie J, Swinton D, Hattman S Mol Cell Biol. 1984; 4(5):985-8.

PMID: 6374428 PMC: 368855. DOI: 10.1128/mcb.4.5.985-988.1984.

References

NEIMARK H . Division of mycoplasmas into subgroups. J Gen Microbiol. 1970; 63(2):249-63. DOI: 10.1099/00221287-63-2-249. View

Liss A, Maniloff J . Isolation of Mycoplasmatales viruses and characterization of MVL1, MVL52, and MVG51. Science. 1971; 173(3998):725-7. DOI: 10.1126/science.173.3998.725. View

GOURLAY R . Mycoplasmatales virus-laidlawii 2, a new virus isolated from Acholeplasma laidlawii. J Gen Virol. 1971; 12(1):65-7. DOI: 10.1099/0022-1317-12-1-65. View

Singhal R . Ion-exlusion chromatography: analysis and isolation of nucleic acid components, and influence of separation parameters. Arch Biochem Biophys. 1972; 152(2):800-10. DOI: 10.1016/0003-9861(72)90276-7. View

Liss A, Maniloff J . Infection of Acholeplasma laidlawii by MVL51 virus. Virology. 1973; 55(1):118-26. DOI: 10.1016/s0042-6822(73)81013-x. View

Pirrotta V . Two restriction endonucleases from Bacillus globiggi. Nucleic Acids Res. 1976; 3(7):1747-60. PMC: 343033. DOI: 10.1093/nar/3.7.1747. View

Sussenbach J, MONFOORT C, Schiphof R, Stobberingh E . A restriction endonuclease from Staphylococcus aureus. Nucleic Acids Res. 1976; 3(11):3193-202. PMC: 343162. DOI: 10.1093/nar/3.11.3193. View

Razin S . The mycoplasmas. Microbiol Rev. 1978; 42(2):414-70. PMC: 281436. DOI: 10.1128/mr.42.2.414-470.1978. View

Bingham A, Atkinson T, Sciaky D, Roberts R . A specific endonuclease from Bacillus caldolyticus. Nucleic Acids Res. 1978; 5(10):3457-67. PMC: 342687. DOI: 10.1093/nar/5.10.3457. View

10.

Nowak J, Maniloff J . Physical characterization of the superhelical DNA genome of an enveloped mycoplasmavirus. J Virol. 1979; 29(1):374-80. PMC: 353134. DOI: 10.1128/JVI.29.1.374-380.1979. View

11.

Dreiseikelmann B, Eichenlaub R, Wackernagel W . The effect of differential methylation by Escherichia coli of plasmid DNA and phage T7 and lambda DNA on the cleavage by restriction endonuclease MboI from Moraxella bovis. Biochim Biophys Acta. 1979; 562(3):418-28. DOI: 10.1016/0005-2787(79)90105-9. View

12.

Woese C, Maniloff J, Zablen L . Phylogenetic analysis of the mycoplasmas. Proc Natl Acad Sci U S A. 1980; 77(1):494-8. PMC: 348298. DOI: 10.1073/pnas.77.1.494. View

13.

Razin A, Razin S . Methylated bases in mycoplasmal DNA. Nucleic Acids Res. 1980; 8(6):1383-90. PMC: 323998. DOI: 10.1093/nar/8.6.1383. View

14.

Roberts R . Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 1981; 9(1):r75-96. PMC: 326683. DOI: 10.1093/nar/9.1.213-c. View

15.

Streeck R . Single-strand and double-strand cleavage at half-modified and fully modified recognition sites for the restriction nucleases Sau3a and Taqi. Gene. 1980; 12(3-4):267-75. DOI: 10.1016/0378-1119(80)90109-2. View