Characterization of Secretin and Vasoactive Intestinal Peptide Receptors in Rat Pancreatic Plasma Membranes Using the Native Peptides, Secretin-(7-27) and Five Secretin Analogues

Overview

Journal Digestion

Specialty Gastroenterology

Date 1982 Jan 1

PMID 6286393

Citations 4

Authors

P Robberecht

M Waelbroeck

M Noyer

P Chatelain

P De Neef

W Konig

J Christophe

Affiliations

Soon will be listed here.

Abstract

A comparison has been made of the ability of vasoactive intestinal peptide (VIP), secretin, secretin analogues, and secretin-(7-27) to stimulate adenylate cyclase in rat pancreatic plasma membranes. A parallel study of the capacity of peptides of the VIP-secretin family to compete with 125I-VIP for binding to the same plasma membranes was conducted. This allowed a classification of VIP-secretin receptors into three subtypes: (1) VIP-preferring receptors; (2) high-affinity secretin receptors, and (3) low-affinity secretin receptors. The properties of secretin at high-affinity secretin receptors were likely to reflect a contribution of membranes from centroacinar and duct cells.

Citing Articles

Secretin: Should we revisit its metabolic outcomes?.

St-Pierre D, Broglio F J Endocrinol Invest. 2010; 33(4):266-75.

PMID: 20503484 DOI: 10.1007/BF03345791.

Topographical distribution of the secretin- and VIP-stimulated adenylate cyclase system in the heart of five animal species.

Chatelain P, Robberecht P, Waelbroeck M, De Neef P, CAMUS J, Huu A Pflugers Arch. 1983; 397(2):100-5.

PMID: 6866728 DOI: 10.1007/BF00582046.

Secretin induces rapid increases in inositol trisphosphate, cytosolic Ca2+ and diacylglycerol as well as cyclic AMP in rat pancreatic acini.

Trimble E, Bruzzone R, Biden T, Farese R Biochem J. 1986; 239(2):257-61.

PMID: 3028367 PMC: 1147275. DOI: 10.1042/bj2390257.

Secretin stimulates cyclic AMP and inositol trisphosphate production in rat pancreatic acinar tissue by two fully independent mechanisms.

Trimble E, Bruzzone R, Biden T, Meehan C, Andreu D, MERRIFIELD R Proc Natl Acad Sci U S A. 1987; 84(10):3146-50.

PMID: 2437575 PMC: 304825. DOI: 10.1073/pnas.84.10.3146.