Identification and Characterization of a Group of Discrete Initiated Oligonucleotides Transcribed in Vitro from the 3' Terminus of the N-gene of Vesicular Stomatitis Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1982 Jun 1

PMID 6285003

Citations 14

Authors

D F Pinney

S U Emerson

Affiliations

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Abstract

Four triphosphate-initiated oligonucleotides, 11 to 14 bases long, produced by in vitro transcription of vesicular stomatitis virus were identified as the uncapped 5' sequences of N-gene mRNA. Characterization of these oligonucleotides reveals that they are continually produced stable transcripts that do not remain template bound. Under the conditions used, the oligonucleotide transcripts were produced at 8 to 10 times the molar amount of leader, suggesting that the N-gene mRNA is internally initiated.

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