A Chromosomally Located Transposon in Pseudomonas Aeruginosa

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1982 Aug 1

PMID 6284702

Citations 23

Authors

M I Sinclair

B W Holloway

Affiliations

Soon will be listed here.

Abstract

A new transposon, Tn2521, coding for carbenicillin, streptomycin, spectinomycin, and sulfanilamide resistance, has been identified in Pseudomonas aeruginosa. The transposon occurs naturally in the chromosome of clinical strains of P. aeruginosa isolated in geographically separated hospitals. This has been demonstrated by its transductional linkage to the pur-136 marker and also by Southern hybridization. Tn2521 is 6.8 kilobases, can transpose from the chromosome to both IncP-1 and IncP-2 plasmid genomes, and has a pattern of restriction endonuclease sites unlike that of any previously described transposon. The carbenicillin resistance carried by Tn2521 is due to the PSE-4 type of beta-lactamase.

Citing Articles

Tn, a Carrier of Tn Family Transposons, Occurs in the Chromosome and in a Genomic Island of Clinical Strains.

Rajabal V, Stanisich V, Petrovski S Microorganisms. 2020; 8(12).

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Integrons: Vehicles and pathways for horizontal dissemination in bacteria.

Domingues S, da Silva G, Nielsen K Mob Genet Elements. 2013; 2(5):211-223.

PMID: 23550063 PMC: 3575428. DOI: 10.4161/mge.22967.

Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.

Domingues S, Harms K, Fricke W, Johnsen P, da Silva G, Nielsen K PLoS Pathog. 2012; 8(8):e1002837.

PMID: 22876180 PMC: 3410848. DOI: 10.1371/journal.ppat.1002837.

Tn502 and Tn512 are res site hunters that provide evidence of resolvase-independent transposition to random sites.

Petrovski S, Stanisich V J Bacteriol. 2010; 192(7):1865-74.

PMID: 20118251 PMC: 2838034. DOI: 10.1128/JB.01322-09.

Class 1 integrons potentially predating the association with tn402-like transposition genes are present in a sediment microbial community.

Stokes H, Nesbo C, Holley M, Bahl M, Gillings M, Boucher Y J Bacteriol. 2006; 188(16):5722-30.

PMID: 16885440 PMC: 1540074. DOI: 10.1128/JB.01950-05.

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