Electron Transport-linked Proton Translocation at Nitrite Reduction in Campylobacter Sputorum Subspecies Bubulus

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 1982 Mar 1

PMID 6280634

Citations 7

Authors

W de Vries

H G Niekus

H van Berchum

A H Stouthamer

Affiliations

Soon will be listed here.

Abstract

Campylobacter sputorum subspecies bubulus contains a membrane-bound nitrite reductase which catalyses the six-electron reduction of nitrite to ammonia. Formate and L-lactate are used as hydrogen donors. Cells of C. sputorum grown with nitrate or nitrite contain cytochromes of the b- and c-type and a carbon monoxide-binding cytochrome c. In addition, a special membrane-bound carbon monoxide-binding pigment is found. Nitrite reduction with formate or L-lactate as a hydrogen donor is strongly inhibited by 2-n-heptyl-4-hydroxyquinoline-N-oxide (HQNO). Nitrite reduction by bacterial suspensions with lactate as a hydrogen donor is strongly inhibited by carbonylcyanide-m-chlorophenylhydrazone (CCCP) whereas nitrite reduction with formate as a hydrogen donor is not inhibited at all. Leads to H+/O values and leads to H+/NO-2 values were measured with ascorbate + N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD), formate (in the absence and presence of carbonic anhydrase) and L-lactate as a hydrogen donor. The results are summarized in a scheme for electron transport from formate or lactate to oxygen or nitrite which shows a periplasmic orientation of formate dehydrogenase and nitrite reductase and a cytoplasmic orientation of lactate dehydrogenase and oxygen reduction, and which shows proton translocation with a leads to H+/2e value of 2.0. The leads to H+/O and leads to H+/NO-2 values predicted by this scheme are in good agreement with the experimental values.

Citing Articles

Fermentative Bacteria Influence the Competition between Denitrifiers and DNRA Bacteria.

van den Berg E, Elisario M, Kuenen J, Kleerebezem R, van Loosdrecht M Front Microbiol. 2017; 8:1684.

PMID: 28928722 PMC: 5591879. DOI: 10.3389/fmicb.2017.01684.

Respiration and growth of Shewanella oneidensis MR-1 using vanadate as the sole electron acceptor.

Carpentier W, Smet L, Van Beeumen J, Brige A J Bacteriol. 2005; 187(10):3293-301.

PMID: 15866913 PMC: 1111990. DOI: 10.1128/JB.187.10.3293-3301.2005.

Aerobic denitrification--old wine in new bottles?.

Robertson L, Kuenen J Antonie Van Leeuwenhoek. 1984; 50(5-6):525-44.

PMID: 6397132 DOI: 10.1007/BF02386224.

Localization of hydrogenase and nitrate reductase in Campylobacter sputorum subsp. bubulus.

de Vries W, van Berchum H, Stouthamer A Antonie Van Leeuwenhoek. 1984; 50(1):63-73.

PMID: 6372687 DOI: 10.1007/BF00404908.

Putative signal peptide on the small subunit of the periplasmic hydrogenase from Desulfovibrio vulgaris.

Prickril B, Czechowski M, Przybyla A, Peck Jr H, LeGall J J Bacteriol. 1986; 167(2):722-5.

PMID: 3525521 PMC: 212951. DOI: 10.1128/jb.167.2.722-725.1986.

References

Scholes P, Smith L . Composition and properties of the membrane-bound respiratory chain system of Micrococcus denitrificans. Biochim Biophys Acta. 1968; 153(2):363-75. DOI: 10.1016/0005-2728(68)90081-9. View

Wikstrom M, Krab K . Proton-pumping cytochrome c oxidase. Biochim Biophys Acta. 1979; 549(2):177-22. DOI: 10.1016/0304-4173(79)90014-4. View

Boogerd F, van Verseveld H, Stouthamer A . Respiration-driven proton translocation with nitrite and nitrous oxide in Paracoccus denitrificans. Biochim Biophys Acta. 1981; 638(2):181-91. DOI: 10.1016/0005-2728(81)90226-7. View

Steenkamp D, Peck Jr H . Proton translocation associated with nitrite respiration in Desulfovibrio desulfuricans. J Biol Chem. 1981; 256(11):5450-8. View

Laanbroek H, Veldkamp H . Growth yield and energy generation in anaerobically-grown Campylobacter spec. Arch Microbiol. 1979; 120(1):47-51. DOI: 10.1007/BF00413272. View

Abou-Jaoude A, PASCAL M, Chippaux M . Formate-nitrite reduction in Escherichia coli K12. 2. Identification of components involved in the electron transfer. Eur J Biochem. 1979; 95(2):315-21. DOI: 10.1111/j.1432-1033.1979.tb12967.x. View

Meijer E, van der Zwaan J, Wever R, Stouthamer A . Anaerobic respiration and energy conservation in Paracoccus denitrificans. Functioning of iron-sulfur centers and the uncoupling effect of nitrite. Eur J Biochem. 1979; 96(1):69-76. DOI: 10.1111/j.1432-1033.1979.tb13014.x. View

Newton N . The two-haem nitrite reductase of Micrococcus denitrificans. Biochim Biophys Acta. 1969; 185(2):316-31. DOI: 10.1016/0005-2744(69)90425-2. View

Inderlied C, Delwiche E . Nitrate reduction and the growth of Veillonella alcalescens. J Bacteriol. 1973; 114(3):1206-12. PMC: 285383. DOI: 10.1128/jb.114.3.1206-1212.1973. View

10.

de Vries W, Niekus H, Boellaard M, Stouthamer A . Growth yields and energy generation by Campylobacter sputorum subspecies bubulus during growth in continuous culture with different hydrogen acceptors. Arch Microbiol. 1980; 124(2-3):221-7. DOI: 10.1007/BF00427730. View

11.

LeGall J, Payne W, Morgan T, DerVartanian D . On the purification of nitrite reductase from Thiobacillus denitrificans and its reaction with nitrite under reducing conditions. Biochem Biophys Res Commun. 1979; 87(2):355-62. DOI: 10.1016/0006-291x(79)91804-7. View

12.

Kemp J, Atkinson D . Nitrite reductase of Escherichia coli specific for reduced nicotinamide adenine dinucleotide. J Bacteriol. 1966; 92(3):628-34. PMC: 276301. DOI: 10.1128/jb.92.3.628-634.1966. View

13.

Steenkamp D, Peck Jr H . The association of hydrogenase and dithionite reductase activities with the nitrite reductase of Desulfovibrio desulfuricans. Biochem Biophys Res Commun. 1980; 94(1):41-8. DOI: 10.1016/s0006-291x(80)80184-7. View

14.

Hasan S, Hall J . The physiological function of nitrate reduction in Clostridium perfringens. J Gen Microbiol. 1975; 87(1):120-8. DOI: 10.1099/00221287-87-1-120. View

15.

HADJIPETROU L, Stouthamer A . ENERGY PRODUCTION DURING NITRATE RESPIRATION BY AEROBACTER AEROGENES. J Gen Microbiol. 1965; 38:29-34. DOI: 10.1099/00221287-38-1-29. View

16.

Niekus H, van Doorn E, Stouthamer A . Oxygen consumption by Campylobacter sputorum subspecies Bubulus with formate as substrate. Arch Microbiol. 1980; 127(2):137-43. DOI: 10.1007/BF00428017. View

17.

Thauer R, Jungermann K, Decker K . Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev. 1977; 41(1):100-80. PMC: 413997. DOI: 10.1128/br.41.1.100-180.1977. View

18.

Abou-Jaoude A, Chippaux M, PASCAL M . Formate-nitrite reduction in Escherchia coli K12. 1. Physiological study of the system. Eur J Biochem. 1979; 95(2):309-14. DOI: 10.1111/j.1432-1033.1979.tb12966.x. View

19.

Scholes P, Mitchell P . Respiration-driven proton translocation in Micrococcus denitrificans. J Bioenerg. 1971; 1(3):309-23. DOI: 10.1007/BF01516290. View

20.

Yoshinari T . N2O reduction by Vibrio succinogenes. Appl Environ Microbiol. 1980; 39(1):81-4. PMC: 291287. DOI: 10.1128/aem.39.1.81-84.1980. View