Study on Conformational States of Escherichia Coli TRNAPhe in Solution by a Modulation-free ESR-spectrometer

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1982 Feb 11

PMID 6278435

Citations 1

Authors

G N Bondarev

V V Isaev-Ivanov

S V Kirillov

A R Kleiner

A F Lepekhin

V B Odinzov

V N Fomichev

Affiliations

Soon will be listed here.

Abstract

A modulation free Electron Spin Resonance spectrometer was used for the registration of spectral absorption lines of a spin-labeled Escherichia coli phenylalanine tRNA in solution with low (less than 0.1%) line shape distortion. The analysis of line shape of two different spin-labels introduced into position 8 revealed that phenylalanine tRNA in solution exists as a mixture of two conformers, the equilibria between conformers being dependent on pH, concentration of magnesium and functional state of tRNA (deacylated, aminoacylated or peptidylated). There are no overall structural rearrangements upon aminoacylation or peptidylation of tRNA. The observed small changes of spectral line shape can be assigned to shifts in conformational equilibria.

Citing Articles

Site-directed spin labeling studies on nucleic acid structure and dynamics.

Sowa G, Qin P Prog Nucleic Acid Res Mol Biol. 2008; 82:147-97.

PMID: 18929141 PMC: 2766621. DOI: 10.1016/S0079-6603(08)00005-6.

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