Structure, Stability, Methylation, Expression and Glucocorticoid Induction of Endogenous and Transfected Proviral Genes of Mouse Mammary Tumor Virus in Mouse Fibroblasts

Overview

Journal Gene

Specialty Molecular Biology

Date 1981 Dec 1

PMID 6277735

Citations 10

Authors

N E Hynes

U Rahmsdorf

N Kennedy

L Fabiani

R Michalides

R Nusse

B Groner

Affiliations

Soon will be listed here.

Abstract

A sequence of mouse genomic DNA containing an endogenous mouse mammary tumor virus (MMTV) provirus, which was isolated by molecular cloning in lambda vector phage, has been reintroduced into cultured mouse L cells, using the thymidine-kinase cotransfection technique. Individual cell clones that acquired the transfected MMTV proviral DNA have been isolated. The transfected DNA remains stable in these cells and does not become methylated. It is transcribed into MMTV RNA, And the transcription is stimulated by glucocorticoid hormones.

Citing Articles

The chromosomal integration site determines the tissue-specific methylation of mouse mammary tumour virus proviral genes.

Gunzburg W, Groner B EMBO J. 1984; 3(5):1129-35.

PMID: 6329738 PMC: 557485. DOI: 10.1002/j.1460-2075.1984.tb01941.x.

Increased concentration of an indigenous proviral mouse mammary tumor virus long terminal repeat-containing transcript is associated with neoplastic transformation of mammary epithelium in C3H/Sm mice.

Graham D, Medina D, Smith G J Virol. 1984; 49(3):819-27.

PMID: 6321767 PMC: 255543. DOI: 10.1128/JVI.49.3.819-827.1984.

Transcription initiation of transfected mouse mammary tumor virus LTR DNA is regulated by glucocorticoid hormones.

Groner B, Hynes N, Rahmsdorf U, Ponta H Nucleic Acids Res. 1983; 11(14):4713-25.

PMID: 6308557 PMC: 326081. DOI: 10.1093/nar/11.14.4713.

Subfragments of the large terminal repeat cause glucocorticoid-responsive expression of mouse mammary tumor virus and of an adjacent gene.

Hynes N, van Ooyen A, Kennedy N, Herrlich P, Ponta H, Groner B Proc Natl Acad Sci U S A. 1983; 80(12):3637-41.

PMID: 6304728 PMC: 394105. DOI: 10.1073/pnas.80.12.3637.

Cloned mouse mammary tumor virus DNA exhibits glucocorticoid-dependent expression in simian virus 40-transformed mink cells.

Owen D, DIGGELMANN H J Virol. 1983; 45(1):148-54.

PMID: 6296429 PMC: 256396. DOI: 10.1128/JVI.45.1.148-154.1983.