Construction of Hybrid Plasmids Containing the LysA Gene of Escherichia Coli: Studies of Expression in Escherichia Coli and Saccharomyces Cerevisiae

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1981 Jan 1

PMID 6272062

Citations 11

Authors

J Chenais

C Richaud

J Ronceray

H Cherest

J C PATTE

Affiliations

Soon will be listed here.

Abstract

The lysA gene of Escherichia coli has been cloned from a lambda transducing phage on various plasmids, present in different copy numbers in bacterial cells. Synthesis of the product of this gene, diaminopimelate (DAP)-decarboxylase, and its regulation have been studied. Expression does not follow a simple gene dosage effect, maximal expression already being obtained with a six-copy plasmid. This result suggests that either a positive or an autogenous regulatory mechanism is involved. We also used one of the hybrid plasmids to look for expression of the bacterial lysA gene in Saccharomyces cerevisiae. The results indicate that the product of the E. coli gene is not actively translated in yeast.

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References

Sussman R, Jacob F . [On a thermosensitive repression system in the Escherichia coli lambda bacteriophage]. C R Hebd Seances Acad Sci. 1962; 254:1517-9. View

Guerry P, Leblanc D, Falkow S . General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973; 116(2):1064-6. PMC: 285495. DOI: 10.1128/jb.116.2.1064-1066.1973. View

Bhattacharjee J, Sinha A . Relationship among the genes, enzymes, and intermediates of the biosynthetic pathway of lysine in Saccharomyces. Mol Gen Genet. 1972; 115(1):26-30. DOI: 10.1007/BF00272214. View

STACEY K, Simson E . IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI. J Bacteriol. 1965; 90:554-5. PMC: 315681. DOI: 10.1128/jb.90.2.554-555.1965. View

Clewell D . Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972; 110(2):667-76. PMC: 247463. DOI: 10.1128/jb.110.2.667-676.1972. View

Galzy P, SLONIMSKI P . [Development of the enzymatic constitution of yeast cultivated on lactic acid or on glucose as sole source of carbon]. C R Hebd Seances Acad Sci. 1957; 245(26):2556-8. View

Cossart P, Katinka M, Yaniv M, Saint Girons I, COHEN G . Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes. Mol Gen Genet. 1979; 175(1):39-44. DOI: 10.1007/BF00267853. View

Rood J, Sneddon M, Morrison J . Instability in tyrR strains of plasmids carrying the tyrosine operon: isolation and characterization of plasmid derivatives with insertions or deletions. J Bacteriol. 1980; 144(2):552-9. PMC: 294702. DOI: 10.1128/jb.144.2.552-559.1980. View

Cohen S, Chang A, Hsu L . Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972; 69(8):2110-4. PMC: 426879. DOI: 10.1073/pnas.69.8.2110. View

10.

Taylor A, Trotter C . Revised linkage map of Escherichia coli. Bacteriol Rev. 1967; 31(4):332-53. PMC: 408288. DOI: 10.1128/br.31.4.332-353.1967. View

11.

Nordstrom K, Ingram L, Lundback A . Mutations in R factors of Escherichia coli causing an increased number of R-factor copies per chromosome. J Bacteriol. 1972; 110(2):562-9. PMC: 247449. DOI: 10.1128/jb.110.2.562-569.1972. View

12.

Nagahari K, Tanaka T, Hishinuma F, Kuroda M, Sakaguchi K . Control of tryptophan synthetase amplified by varying the numbers of composite plasmids in Escherichia coli cells. Gene. 1977; 1(2):141-52. DOI: 10.1016/0378-1119(77)90025-7. View

13.

Guerineau M, Grandchamp C, Paoletti C, SLONIMSKI P . Characterization of a new class of circular DNA molecules in yeast. Biochem Biophys Res Commun. 1971; 42(3):550-7. DOI: 10.1016/0006-291x(71)90406-2. View

14.

Kelleher Jr R, Heggeness M . Repression of diaminopimelic acid decarboxylase in Escherichia coli: gene dosage effects and escape synthesis. J Bacteriol. 1976; 125(1):376-8. PMC: 233376. DOI: 10.1128/jb.125.1.376-378.1976. View

15.

Hinnen A, Hicks J, Fink G . Transformation of yeast. Proc Natl Acad Sci U S A. 1978; 75(4):1929-33. PMC: 392455. DOI: 10.1073/pnas.75.4.1929. View

16.

TIKCHONENKO T, Faizullin L, Kalinin V, Granovsky N, Naroditsky B . Construction and properties of hybrid plasmids carrying the E. coli gal operon. Gene. 1979; 7(2):109-19. DOI: 10.1016/0378-1119(79)90027-1. View

17.

Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View

18.

Novick R . Micro-iodometric assay for penicillinase. Biochem J. 1962; 83:236-40. PMC: 1243538. DOI: 10.1042/bj0830236. View

19.

CHEVALLIER M, Aigle M . Qualitative detection of penicillinase produced by yeast strains carrying chimeric yeast-coli plasmids. FEBS Lett. 1979; 108(1):179-80. DOI: 10.1016/0014-5793(79)81204-1. View

20.

PATTE J, Morand P, Boy E, Richaud C, Borne F . The relA locus and the regulation of lysine biosynthesis in Escherichia coli. Mol Gen Genet. 1980; 179(2):319-25. DOI: 10.1007/BF00425459. View