Identification of the E. Coli GroNB(nusB) Gene Product

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1981 Jan 1

PMID 6272060

Citations 8

Authors

J Swindle

J Ajioka

C Georgopoulos

Affiliations

Soon will be listed here.

Abstract

The E. coli groNB(nusB) gene product has been previously shown to be necessary for bacteriophage lambda N protein function. The product of the groNB gene has been identified on SDS polyacrylamide gels after infection of UV-irradiated E. coli cells with various lambda groNB+ transducing phage derivatives. It is a polypeptide with an apparent molecular weight of 14,000 daltons. Transducing phage carrying either a deletion or an amber mutation in the groNB gene fail to synthesize the 14,000-Mr polypeptide chain upon infection of a sup+ host. However, am+ revertants of the lambda groNBam phage do induce the synthesis of the polypeptide.

Citing Articles

Molecular cloning and nucleotide sequencing of the nusB gene of E. coli.

Ishii S, Hatada E, Maekawa T, Imamoto F Nucleic Acids Res. 1984; 12(12):4987-95.

PMID: 6330694 PMC: 318893. DOI: 10.1093/nar/12.12.4987.

The nucleotide sequence of the Escherichia coli K12 nusB (groNB) gene.

Swindle J, Ajioka J, Dawson D, Myers R, Carroll D, Georgopoulos C Nucleic Acids Res. 1984; 12(12):4977-85.

PMID: 6330693 PMC: 318892. DOI: 10.1093/nar/12.12.4977.

Identification of the nusB gene product of Escherichia coli.

Strauch M, Friedman D Mol Gen Genet. 1981; 182(3):498-501.

PMID: 6272065 DOI: 10.1007/BF00293941.

Interactions of bacteriophage and host macromolecules in the growth of bacteriophage lambda.

Friedman D, Olson E, Georgopoulos C, Tilly K, Herskowitz I, Banuett F Microbiol Rev. 1984; 48(4):299-325.

PMID: 6240590 PMC: 373221. DOI: 10.1128/mr.48.4.299-325.1984.

nusB: a protein factor necessary for transcription antitermination in vitro by phage lambda N gene product.

Ghosh B, Das A Proc Natl Acad Sci U S A. 1984; 81(20):6305-9.

PMID: 6093096 PMC: 391912. DOI: 10.1073/pnas.81.20.6305.

References

Rosenberg M, Court D . Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979; 13:319-53. DOI: 10.1146/annurev.ge.13.120179.001535. View

Georgopoulos C, Swindle J, KEPPEL F, BALLIVET M, Bisig R, Eisen H . Studies on the E. coli groNB (nusB) gene which affects bacteriophage lambda N gene function. Mol Gen Genet. 1980; 179(1):55-61. DOI: 10.1007/BF00268446. View

Nakamura Y, Kurihara T, Saito H, Uchida H . Sigma subunit of Escherichia coli RNA polymerase affects the function of lambda N gene. Proc Natl Acad Sci U S A. 1979; 76(9):4593-7. PMC: 411625. DOI: 10.1073/pnas.76.9.4593. View

GREENBLATT J, Li J, Adhya S, Friedman D, Baron L, Redfield B . L factor that is required for beta-galactosidase synthesis is the nusA gene product involved in transcription termination. Proc Natl Acad Sci U S A. 1980; 77(4):1991-4. PMC: 348636. DOI: 10.1073/pnas.77.4.1991. View

Salstrom J, Fiandt M, SZYBALSKI W . The site controlling the specificity of N action is outside the promoter-operator region: a triple hybrid phage lambda N21 imm434nin5. Gene. 1979; 5(4):305-27. DOI: 10.1016/0378-1119(79)90105-7. View

GREENBLATT J, Malnoe P, Li J . Purification of the gene N transcription anti-termination protein of bacteriophage lambda. J Biol Chem. 1980; 255(4):1465-70. View

Sternberg N . A class of rifR RNA polymerase mutations that interferes with the expression of coliphage lambda late gene. Virology. 1976; 73(1):139-54. DOI: 10.1016/0042-6822(76)90068-4. View

Ghysen A, Pironio M . Relationship between the N function of bacteriophage lambda and host RNA polymerase. J Mol Biol. 1972; 65(2):259-72. DOI: 10.1016/0022-2836(72)90281-1. View

KEPPEL F, Georgopoulos C, Eisen H . Host interference with expression of the lambda N gene product. Biochimie. 1974; 56(11-12):1505-9. DOI: 10.1016/s0300-9084(75)80273-2. View

10.

Friedman D, Wilgus G, Mural R . Gene N regulator function of phage lambda immun21: evidence that a site of N action differs from a site of N recognition. J Mol Biol. 1973; 81(4):505-16. DOI: 10.1016/0022-2836(73)90519-6. View

11.

FOWLER R, Degnen G, Cox E . Mutational specificity of a conditional Escherichia coli mutator, mutD5. Mol Gen Genet. 1974; 133(3):179-91. DOI: 10.1007/BF00267667. View

12.

Adhya S, Gottesman M . Control of transcription termination. Annu Rev Biochem. 1978; 47:967-96. DOI: 10.1146/annurev.bi.47.070178.004535. View

13.

Carroll D, Ajioka R, Georgopoulos C . Bacteriophage lambda cloning vehicles for studies of genetic recombination. Gene. 1980; 10(3):261-71. DOI: 10.1016/0378-1119(80)90055-4. View

14.

Kung H, Spears C, Weissbach H . Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase. J Biol Chem. 1975; 250(4):1556-62. View

15.

Friedman D, Baumann M, Baron L . Cooperative effects of bacterial mutations affecting lambda N gene expression. I. Isolation and characterization of a nusB mutant. Virology. 1976; 73(1):119-27. DOI: 10.1016/0042-6822(76)90066-0. View

16.

Salstrom J, SZYBALSKI W . Coliphage lambdanutL-: a unique class of mutants defective in the site of gene N product utilization for antitermination of leftward transcription. J Mol Biol. 1978; 124(1):195-221. DOI: 10.1016/0022-2836(78)90156-0. View

17.

GREENBLATT J . Regulation of transcription termination by the N gene protein of bacteriophage lambda. Cell. 1981; 24(1):8-9. DOI: 10.1016/0092-8674(81)90495-5. View

18.

Roberts J . Promoter mutation in vitro. Nature. 1969; 223(5205):480-2. DOI: 10.1038/223480a0. View

19.

Friedman D, Jolly C, Mural R . Interference with the expression of the N gene function of phage lambda in a mutant of Escherichia coli. Virology. 1973; 51(1):216-26. DOI: 10.1016/0042-6822(73)90381-4. View

20.

Friedman D, Schauer A, Baumann M, Baron L, Adhya S . Evidence that ribosomal protein S10 participates in control of transcription termination. Proc Natl Acad Sci U S A. 1981; 78(2):1115-8. PMC: 319957. DOI: 10.1073/pnas.78.2.1115. View