Characterization of a Macrolide, Lincosamide, and Streptogramin Resistance Plasmid in Staphylococcus Epidermidis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1981 Nov 1

PMID 6271735

Citations 13

Authors

J T Parisi

J Robbins

B C Lampson

D W Hecht

Affiliations

Soon will be listed here.

Abstract

A strain of Staphylococcus epidermidis was transduced to erythromycin resistance, and all of the transductants exhibited the macrolide, lincosamide, streptogramin B resistance phenotype. Curing and antibiotic disk studies also indicated that these resistances were controlled by a single plasmid determinant and were constitutive. Agarose gel electrophoresis of plasmid deoxyribonucleic acid (DNA) from donor, cured, and transduced strains showed that a single plasmid was responsible. This plasmid, designated pNE131, was examined for sequence homology to two other plasmids, pE194 and p1258, from Staphylococcus aureus, which also code for erythromycin resistance. DNA from plasmids pNE131 and pE194 hybridized with one another, but no extensive homology to pI258 with either pNE131 or pE194 was found. Restriction endonuclease digests of pNE131 and pE194 showed no common fragments. However, sequence homology was localized to the nucleotides in pE194 that code for the 29,000-dalton protein responsible for erythromycin resistance. pNE131 was calculated to have 2,220 base pairs and is the smallest naturally occurring plasmid with a known function yet reported in S. epidermidis.

Citing Articles

Prevalence of erm gene classes in erythromycin-resistant Staphylococcus aureus strains isolated between 1959 and 1988.

Westh H, Hougaard D, Vuust J, Rosdahl V Antimicrob Agents Chemother. 1995; 39(2):369-73.

PMID: 7726500 PMC: 162545. DOI: 10.1128/AAC.39.2.369.

Novel mechanism for plasmid-mediated erythromycin resistance by pNE24 from Staphylococcus epidermidis.

Lampson B, von David W, Parisi J Antimicrob Agents Chemother. 1986; 30(5):653-8.

PMID: 3800341 PMC: 176508. DOI: 10.1128/AAC.30.5.653.

Comparison of epidemiologic markers for Staphylococcus epidermidis.

Parisi J, Lampson B, Hoover D, Khan J J Clin Microbiol. 1986; 24(1):56-60.

PMID: 3722366 PMC: 268831. DOI: 10.1128/jcm.24.1.56-60.1986.

Novel mechanisms of resistance to lincosamides in Staphylococcus and Arthrobacter spp.

Quiros L, Fidalgo S, Mendez F, Hardisson C, Salas J Antimicrob Agents Chemother. 1988; 32(4):420-5.

PMID: 3377455 PMC: 172193. DOI: 10.1128/AAC.32.4.420.

Prevalence of macrolides-lincosamides-streptogramin B resistance and erm gene classes among clinical strains of staphylococci and streptococci.

Jenssen W, Thakker-Varia S, DUBIN D, Weinstein M Antimicrob Agents Chemother. 1987; 31(6):883-8.

PMID: 3113328 PMC: 284204. DOI: 10.1128/AAC.31.6.883.

References

Denhardt D . A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966; 23(5):641-6. DOI: 10.1016/0006-291x(66)90447-5. View

Khan S, Novick R . Terminal nucleotide sequences of Tn551, a transposon specifying erythromycin resistance in Staphylococcus aureus: homology with Tn3. Plasmid. 1980; 4(2):148-54. DOI: 10.1016/0147-619x(80)90004-9. View

Cohen S, Miller C . Multiple molecular species of circular R-factor DNA isolated from Escherichia coli. Nature. 1969; 224(5226):1273-7. DOI: 10.1038/2241273a0. View

Lindberg M, Novick R . Plasmid-specific transformation in Staphylococcus aureus. J Bacteriol. 1973; 115(1):139-45. PMC: 246223. DOI: 10.1128/jb.115.1.139-145.1973. View

Parisi J, Talbot Jr H . Improved rapid plate method for the isolation of bacteriophages from lysogenic bacteria. Appl Microbiol. 1974; 28(3):503-4. PMC: 186751. DOI: 10.1128/am.28.3.503-504.1974. View

Maniatis T, Jeffrey A, Kleid D . Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975; 72(3):1184-8. PMC: 432491. DOI: 10.1073/pnas.72.3.1184. View

Males B, Rogers Jr W, Parisi J . Virulence factors of biotypes of Staphylococcus epidermidis from clinical sources. J Clin Microbiol. 1975; 1(3):256-61. PMC: 275050. DOI: 10.1128/jcm.1.3.256-261.1975. View

Witte W . Transfer of drug-resistance-plasmids in mixed cultures of Staphylococci. Zentralbl Bakteriol Orig A. 1977; 237(2-3):147-59. View

Parisi J, Talbot H, Skahan J . Development of a phage typing set for Staphylococcus epidermidis in the United States. Zentralbl Bakteriol Orig A. 1978; 241(1):60-7. View

10.

Weisblum B, Graham M, Gryczan T, Dubnau D . Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J Bacteriol. 1979; 137(1):635-43. PMC: 218492. DOI: 10.1128/jb.137.1.635-643.1979. View

11.

Novick R, Edelman I, Schwesinger M, Gruss A, Swanson E, Pattee P . Genetic translocation in Staphylococcus aureus. Proc Natl Acad Sci U S A. 1979; 76(1):400-4. PMC: 382947. DOI: 10.1073/pnas.76.1.400. View

12.

Macrina F, Kopecko D, Jones K, Ayers D, McCowen S . A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid. 1978; 1(3):417-20. DOI: 10.1016/0147-619x(78)90056-2. View

13.

Iordanescu S, SURDEANU M, Della Latta P, NOVICK R . Incompatibility and molecular relationships between small Staphylococcal plasmids carrying the same resistance marker. Plasmid. 1978; 1(4):468-79. DOI: 10.1016/0147-619x(78)90005-7. View

14.

Novick R, Murphy E, Gryczan T, Baron E, Edelman I . Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps. Plasmid. 1979; 2(1):109-29. DOI: 10.1016/0147-619x(79)90010-6. View

15.

Olson Jr W, Parisi J, Totten P, Baldwin J . Transduction of penicillinase production in Staphylococcus epidermidis and nature of the genetic determinant. Can J Microbiol. 1979; 25(4):508-11. DOI: 10.1139/m79-074. View

16.

Wahl G, Stern M, Stark G . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979; 76(8):3683-7. PMC: 383897. DOI: 10.1073/pnas.76.8.3683. View

17.

Sjostrom J, Lofdahl S, Philipson L . Transformation of Staphylococcus aureus by heterologous plasmids. Plasmid. 1979; 2(4):529-35. DOI: 10.1016/0147-619x(79)90052-0. View

18.

Groves D . Interspecific relationships of antibiotic resistance in Staphylococcus sp.: isolation and comparison of plasmids determining tetracycline resistance in S. aureus and S. epidermidis. Can J Microbiol. 1979; 25(12):1468-75. DOI: 10.1139/m79-227. View

19.

Parisi J, Hecht D . Plasmid profiles in epidemiologic studies of infections by Staphylococcus epidermidis. J Infect Dis. 1980; 141(5):637-43. DOI: 10.1093/infdis/141.5.637. View

20.

Jaffe H, SWEENEY H, Nathan C, Weinstein R, KABINS S, Cohen S . Identity and interspecific transfer of gentamicin-resistance plasmids in Staphylococcus aureus and Staphylococcus epidermidis. J Infect Dis. 1980; 141(6):738-47. DOI: 10.1093/infdis/141.6.738. View