Cloning of a RecA-like Gene of Proteus Mirabilis

Overview

Journal Gene

Specialty Molecular Biology

Date 1981 Sep 1

PMID 6271629

Citations 11

Authors

G Eitner

A S Solonin

V I Tanyashin

Affiliations

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Abstract

A gene of Proteus mirabilis that can substitute for functions of the recA gene of Escherichia coli has been cloned into the plasmid pBR322, using shotgun experiments. The recA-like gene (recAp.m.) has been localized by restriction mapping within a 1.5-Md PstI fragment that is a part of two cloned HindIII fragments of the chromosome of P. mirabilis. The restriction map of the recAp.m. gene differs from that of the recA gene of E. coli. Functionally, the recombinant plasmids containing the recAp.m. gene restore a nearly wild-type level of UV-resistance to several point and deletion mutants in the recA gene of E. coli.

Citing Articles

Interspecies recA protein substitution in Escherichia coli and Proteus mirabilis.

Eitner G, Adler B, Lanzov V, Hofemeister J Mol Gen Genet. 1982; 185(3):481-6.

PMID: 7048026 DOI: 10.1007/BF00334144.

Functional substitution of the recE gene of Bacillus subtilis by the recA gene of Proteus mirabilis.

Eitner G, Manteuffel R, Hofemeister J Mol Gen Genet. 1984; 195(3):516-22.

PMID: 6433153 DOI: 10.1007/BF00341456.

P. mirabilis RecA protein catalyses cleavage of E. coli LexA protein and the lambda repressor in vitro.

West S, Little J Mol Gen Genet. 1984; 194(1-2):111-3.

PMID: 6374373 DOI: 10.1007/BF00383505.

The sfiA11 mutation prevents filamentation in a response to cell wall damage only in a recA+ genetic background.

Cegielska A, Taylor A Mol Gen Genet. 1985; 201(3):537-42.

PMID: 3911030 DOI: 10.1007/BF00331352.

Resistance to chloramphenicol in Proteus mirabilis by expression of a chromosomal gene for chloramphenicol acetyltransferase.

Charles I, Harford S, Brookfield J, Shaw W J Bacteriol. 1985; 164(1):114-22.

PMID: 3900034 PMC: 214218. DOI: 10.1128/jb.164.1.114-122.1985.