Receptor Autoradiography with Tritium-sensitive Film: Potential for Computerized Densitometry

Overview

Journal Neurosci Lett

Specialty Neurology

Date 1981 Sep 1

PMID 6269032

Citations 14

Authors

J M Palacios

D L Niehoff

M J Kuhar

Affiliations

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Abstract

Benzodiazepine receptors mounted tissue sections of brain were localized by autoradiography using a tritium-sensitive sheet film (3H-Ultrofilm, LKB). The use of the film had many advantages compared to a previous technique utilizing emulsion-coated coverslips. A major advantage is the adaptability of the film to microdensitometric techniques for measurement of receptors. The densitometry can be carried out by computerized, color-coded image analysis.

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