Induction and Genetics of Two Alpha-galactosidase Activities in Saccharomyces Cerevisiae

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1981 Jan 1

PMID 6267434

Citations 9

Authors

R G Buckholz

B G ADAMS

Affiliations

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Abstract

The induction of alpha-galactosidase of Saccharomyces cerevisiae was investigated. We have demonstrated the existence of inducible internal and external alpha-galactosidase activities and have studied the relationship between the two alpha-galactosidases by examining a mutant strain which lacks both the internal and external activities. The mutant possesses a mutation in a single locus (mel1-1) which does not affect the synthesis of the other galactose pathway enzymes or the ability of the yeast to grow on media containing only galactose as the carbon source. Genetic studies of the mutant indicate that mel 1-1 is recessive and allelic to the wild tye allele for melibiose fermentation Mel 1.

Citing Articles

Construction of a Stable alpha-Galactosidase-Producing Baker's Yeast Strain.

Liljestrom-Suominen P, Joutsjoki V, Korhola M Appl Environ Microbiol. 1988; 54(1):245-249.

PMID: 16347529 PMC: 202428. DOI: 10.1128/aem.54.1.245-249.1988.

Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon.

Johnston S, Hopper J Proc Natl Acad Sci U S A. 1982; 79(22):6971-5.

PMID: 6294669 PMC: 347256. DOI: 10.1073/pnas.79.22.6971.

Regulation of basal and induced levels of the MEL1 transcript in Saccharomyces cerevisiae.

Hamilton R, Hopper J Mol Cell Biol. 1984; 4(7):1238-45.

PMID: 6209559 PMC: 368904. DOI: 10.1128/mcb.4.7.1238-1245.1984.

Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases.

Bajwa W, Torchia T, Hopper J Mol Cell Biol. 1988; 8(8):3439-47.

PMID: 3062381 PMC: 363581. DOI: 10.1128/mcb.8.8.3439-3447.1988.

Genetic and molecular analysis of the GAL3 gene in the expression of the galactose/melibiose regulon of Saccharomyces cerevisiae.

Torchia T, Hopper J Genetics. 1986; 113(2):229-46.

PMID: 3013721 PMC: 1202836. DOI: 10.1093/genetics/113.2.229.

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