Cloning of Hepatitis A Virus Genome

Overview

Journal J Virol Methods

Specialty Microbiology

Date 1981 Jul 1

PMID 6267095

Citations 7

Authors

K von der Helm

E L Winnacker

F Deinhardt

G Frosner

V Gauss-Muller

B Bayerl

R Scheid

G Siegl

Affiliations

Soon will be listed here.

Abstract

Hepatitis A virus (HAV) was highly purified from faeces. The genomic RNA was transcribed to cDNA and this DNA was then cloned into plasmid pBR 322 at the Pst I site, and clones were selected in presence of tetracycline. Most clones contained inserts which hybridized to HAV-specific RNA isolated from HAV-infected cell cultures derived from a human hepatocellular carcinoma. Two clones expressed low amounts of viral antigens.

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