DNase I Hypersensitive Sites in Drosophila Chromatin Occur at the 5' Ends of Regions of Transcription

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1981 Jan 1

PMID 6264428

Citations 97

Authors

M A Keene

V Corces

K Lowenhaupt

S C Elgin

Affiliations

Soon will be listed here.

Abstract

By using a map of the unique region of DNA encoding the fur small heat-shock proteins of Drosophila melanogaster (hsp 22, hsp 23, hsp 26, and hsp 28), and a simple mapping technique, the positions of the DNase I hypersensitive sites of chromatin in the vicinity of these genes have now been determined. The major chromatin-specific sites occur at the 5' ends of each of the four heat-shock protein genes in embryo nuclei. These genes are not active in the nuclei analyzed but can be quickly induced in these cells by the heat-shock stimulus. The chromatin structure indicated by DNase I hypersensitivity may be a necessary factor in the general mechanism of gene activation.

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