Ordering Tryptophan Synthase Genes of Pseudomonas Aeruginosa by Cloning in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1981 Apr 1

PMID 6260735

Citations 4

Authors

J N Manch

I P Crawford

Affiliations

Soon will be listed here.

Abstract

The Pseudomonas aeruginosa tryptophan synthase genes, trpA and trpB, which are induced by their substrate indoleglycerol phosphate, were cloned along with their controlling region into the BamHI site of pBR322 to produce the 10.7-megadalton plasmid pZAZ5. SalI partial digestion and ligation yielded a smaller plasmid, pZAZ167, with the chromosomal insert reduced in size from 8.1 to 3.4 megadaltons. Both pZAZ5 and pZAZ167 display Pseudomonas-like regulation of the trpA and trpB genes. Deletion of an EcoRI fragment or a BglII fragment from pZAZ167 yielded plasmids pZAZ168 and pZAZ169; the former expresses trpB but not trpA, and the latter has lost both activities. A deleted form of pZAZ5 designated pZAZ101 was obtained by excising a BglII-BamHI segment and religating the trip gene segment in the opposite orientation. This plasmid expresses trpA and trpB constitutively. The physical maps of these plasmids establish the gene order: promoter-trpB-trpA.

Citing Articles

Arginine degradation in Pseudomonas aeruginosa mutants blocked in two arginine catabolic pathways.

Haas D, Matsumoto H, Moretti P, Stalon V, Mercenier A Mol Gen Genet. 1984; 193(3):437-44.

PMID: 6423933 DOI: 10.1007/BF00382081.

Sequence of the Pseudomonas aeruginosa trpI activator gene and relatedness of trpI to other procaryotic regulatory genes.

Chang M, Hadero A, Crawford I J Bacteriol. 1989; 171(1):172-83.

PMID: 2492495 PMC: 209570. DOI: 10.1128/jb.171.1.172-183.1989.

The roles of indoleglycerol phosphate and the TrpI protein in the expression of trpBA from Pseudomonas aeruginosa.

Chang M, Crawford I Nucleic Acids Res. 1990; 18(4):979-88.

PMID: 2107533 PMC: 330353. DOI: 10.1093/nar/18.4.979.

In vitro determination of the effect of indoleglycerol phosphate on the interaction of purified TrpI protein with its DNA-binding sites.

Chang M, Crawford I J Bacteriol. 1991; 173(5):1590-7.

PMID: 1900276 PMC: 207307. DOI: 10.1128/jb.173.5.1590-1597.1991.

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