Test Protocol of an Enzyme-linked Immunosorbent Assay (ELISA) for the Detection of Antibodies Against Bovine Leukosis Virus

Overview

Journal Vet Q

Specialty Veterinary Medicine

Date 1981 Jan 15

PMID 6259771

Citations 3

Authors

A L Gielkens

A A Ressang

J Ijzermann

J Quak

Affiliations

Soon will be listed here.

Abstract

In this communication the test procedure is described for an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against bovine leukosis virus (BLV). Test sera are incubated in polystyrene microtiter plates sensitized with a partly-purified preparation of BLV. Bovine antibodies are detected with anti-species immunoglobulin conjugated to the enzyme horseradish peroxidase, followed by the addition of the enzyme substrate.

Citing Articles

Pharmacological investigation of taxifolin for its therapeutic potential in depression.

Mir M, Khan A, Khan A Heliyon. 2024; 10(9):e30467.

PMID: 38694040 PMC: 11061746. DOI: 10.1016/j.heliyon.2024.e30467.

Heat shock preconditioning mesenchymal stem cells attenuate acute lung injury via reducing NLRP3 inflammasome activation in macrophages.

Lv H, Yuan X, Zhang J, Lu T, Yao J, Zheng J Stem Cell Res Ther. 2021; 12(1):290.

PMID: 34001255 PMC: 8127288. DOI: 10.1186/s13287-021-02328-3.

Applications of enzyme-linked immunosorbent assay in veterinary medicine: a bibliography.

Charan S, Gautam O Vet Res Commun. 1984; 8(4):255-67.

PMID: 6393563 PMC: 7088733. DOI: 10.1007/BF02214720.