Action of Calcium Ions on Spinach (Spinacia Oleracea) Chloroplast Fructose Bisphosphatase and Other Enzymes of the Calvin Cycle

Overview

Journal Biochem J

Specialty Biochemistry

Date 1980 Jun 15

PMID 6258561

Citations 21

Authors

S A Charles

B Halliwell

Affiliations

Soon will be listed here.

Abstract

Thiol-treated spinach (Spinacia oleracea) chloroplast fructose bisphosphatase is powerfully inhibited by Ca2+ non-competitively with respect to its substrate, fructose 1,6-bisphosphate. 500 microM-Ca2+ causes virtually complete inhibition and the Ki is 40 microM. Severe inhibition of sedoheptulose bisphosphatase is also caused by Ca2+. A role for Ca2+ in regulation of the Calvin cycle in spinach chloroplasts is proposed.

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References

Bulychev A, Vredenberg W . Effect of ionophores A23187 and nigericin on the light-induced redistribution of Mg2+, K+ and H+ across the thylakoid membrane. Biochim Biophys Acta. 1976; 449(1):48-58. DOI: 10.1016/0005-2728(76)90006-2. View

Roeske R, Isaac S, King T, Steinrauf L . The binding of barium and calcium ions by the antibiotic beauvericin. Biochem Biophys Res Commun. 1974; 57(3):554-61. DOI: 10.1016/0006-291x(74)90582-8. View

Barr R, Troxel K, Crane F . EGTA, a calcium chelator, inhibits electron transport in photosystem II of spinach chloroplasts at two different sites. Biochem Biophys Res Commun. 1980; 92(1):206-12. DOI: 10.1016/0006-291x(80)91540-5. View

Arnon D . COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949; 24(1):1-15. PMC: 437905. DOI: 10.1104/pp.24.1.1. View

Portis Jr A, Heldt H . Light-dependent changes of the Mg2+ concentration in the stroma in relation to the Mg2+ dependency of CO2 fixation in intact chloroplasts. Biochim Biophys Acta. 1976; 449(3):434-6. DOI: 10.1016/0005-2728(76)90154-7. View

Krause G . Light-induced movement of magnesium ions in intact chloroplasts. Spectroscopic determination with Eriochrome Blue SE. Biochim Biophys Acta. 1977; 460(3):500-10. DOI: 10.1016/0005-2728(77)90088-3. View

Avron M, Gibbs M . Properties of phosphoribulokinase of whole chloroplasts. Plant Physiol. 1974; 53(2):136-9. PMC: 541351. DOI: 10.1104/pp.53.2.136. View

Heber U, Santarius K . Direct and indirect transfer of ATP and ADP across the chloroplast envelope. Z Naturforsch B. 1970; 25(7):718-28. DOI: 10.1515/znb-1970-0714. View

Hind G, Nakatani H, Izawa S . Light-dependent redistribution of ions in suspensions of chloroplast thylakoid membranes. Proc Natl Acad Sci U S A. 1974; 71(4):1484-8. PMC: 388254. DOI: 10.1073/pnas.71.4.1484. View

10.

Prochaska L, Gross E . The effect of 1-ethyl-3(3-dimethylaminopropyl)carbodiimide on calcium binding and associated changes in chloroplast structure and chlorophyll a fluorescence in spinach chloroplasts. Biochim Biophys Acta. 1975; 376(1):126-35. DOI: 10.1016/0005-2728(75)90211-x. View

11.

DAVIS D, Gross E . Protein-protein interactions of light-harvesting pigment protein from spinach chloroplasts. I.Ca-2+ binding and its relation to protein association. Biochim Biophys Acta. 1975; 387(3):557-67. DOI: 10.1016/0005-2728(75)90093-6. View

12.

Hall D . Nomenclature for isolated chloroplasts. Nat New Biol. 1972; 235(56):125-6. DOI: 10.1038/newbio235125a0. View

13.

Nobel P . Light-induced changes in the ionic content of chloroplasts in Pisum sativum. Biochim Biophys Acta. 1969; 172(1):134-43. DOI: 10.1016/0005-2728(69)90098-x. View

14.

Nakatani H, Barber J . An improved method for isolating chloroplasts retaining their outer membranes. Biochim Biophys Acta. 1977; 461(3):500-12. View

15.

Barber J, Mills J, Nicolson J . Studies with cation specific ionophores show that within the intact chloroplast Mg++ acts as the main exchange cation for H+ pumping. FEBS Lett. 1974; 49(1):106-10. DOI: 10.1016/0014-5793(74)80643-5. View

16.

Ben-Hayyim G . Mg2+ translocation across the thylakoid membrane: studies using the ionophore A 23187. Eur J Biochem. 1978; 83(1):99-104. DOI: 10.1111/j.1432-1033.1978.tb12072.x. View

17.

RACKER E, SCHROEDER E . The reductive pentose phosphate cycle. II. Specific C-1 phosphatases for fructose 1,6-diphosphate and sedoheptulose 1,7-diphosphate. Arch Biochem Biophys. 1958; 74(2):326-44. DOI: 10.1016/0003-9861(58)90004-3. View

18.

Charles S, Halliwell B . Properties of freshly purified and thiol-treated spinach chloroplast fructose bisphosphatase. Biochem J. 1980; 185(3):689-93. PMC: 1161446. DOI: 10.1042/bj1850689. View

19.

Werdan K, Heldt H, Milovancev M . The role of pH in the regulation of carbon fixation in the chloroplast stroma. Studies on CO2 fixation in the light and dark. Biochim Biophys Acta. 1975; 396(2):276-92. DOI: 10.1016/0005-2728(75)90041-9. View

20.

Cockburn W, Baldry C, Walker D . Some effects of inorganic phosphate on O2 evolution by isolated chloroplasts. Biochim Biophys Acta. 1967; 143(3):614-24. DOI: 10.1016/0005-2728(67)90067-9. View