Analysis of the Regions Flanking the Human Insulin Gene and Sequence of an Alu Family Member

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1980 Sep 25

PMID 6253909

Citations 58

Authors

G I Bell

R Pictet

W J Rutter

Affiliations

Soon will be listed here.

Abstract

The regions around the human insulin gene have been studied by heteroduplex, hybridization and sequence analysis. These studies indicated that there is a region of heterogeneous length located approximately 700 bp before the 5' end of the gene; and that the 19 kb of cloned DNA which includes the 1430 bp insulin gene as well as 5650 bp before and 11,500 bp after the gene is single copy sequence except for 500 bp located 6000 bp from the 3' end of the gene. This 500 bp segment contains a member of the Alu family of dispersed middle repetitive sequences as well as another less highly repeated homopolymeric segment. The sequence of this region was determined. This Alu repeat is bordered by 19 bp direct repeats and also contains an 83 bp sequence which is present twice. The regions flanking the human and rat I insulin genes were compared by heteroduplex analysis to localize homologous sequences in the flanking regions which could be involved in the regulation of insulin biosynthesis. The homology between the two genes is restricted to the region encoding preproinsulin and a short region of approximately 60 bp flanking the 5' side of the genes.

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References

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Maxam A, Gilbert W . A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977; 74(2):560-4. PMC: 392330. DOI: 10.1073/pnas.74.2.560. View

Valenzuela P, Bell G, Masiarz F, DeGennaro L, Rutter W . Nucleotide sequence of the yeast 5S ribosomal RNA gene and adjacent putative control regions. Nature. 1977; 267(5612):641-3. DOI: 10.1038/267641a0. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Valenzuela P, Venegas A, Weinberg F, Bishop R, Rutter W . Structure of yeast phenylalanine-tRNA genes: an intervening DNA segment within the region coding for the tRNA. Proc Natl Acad Sci U S A. 1978; 75(1):190-4. PMC: 411211. DOI: 10.1073/pnas.75.1.190. View

Bostock C, Gosden J, Mitchell A . Localisation of a male-specific DNA fragment to a sub-region of the human Y chromosome. Nature. 1978; 272(5651):324-8. DOI: 10.1038/272324a0. View

Manuelidis L . Complex and simple sequences in human repeated DNAs. Chromosoma. 1978; 66(1):1-21. DOI: 10.1007/BF00285812. View

Finnegan D, Rubin G, YOUNG M, Hogness D . Repeated gene families in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1978; 42 Pt 2:1053-63. DOI: 10.1101/sqb.1978.042.01.106. View

Cameron J, Loh E, Davis R . Evidence for transposition of dispersed repetitive DNA families in yeast. Cell. 1979; 16(4):739-51. DOI: 10.1016/0092-8674(79)90090-4. View

10.

Potter S, Brorein Jr W, Dunsmuir P, Rubin G . Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell. 1979; 17(2):415-27. DOI: 10.1016/0092-8674(79)90168-5. View

11.

Cooke H, Hindley J . Cloning of human satellite III DNA: different components are on different chromosomes. Nucleic Acids Res. 1979; 6(10):3177-97. PMC: 327928. DOI: 10.1093/nar/6.10.3177. View

12.

Wahl G, Stern M, Stark G . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979; 76(8):3683-7. PMC: 383897. DOI: 10.1073/pnas.76.8.3683. View

13.

Cordell B, Bell G, Tischer E, DeNoto F, Ullrich A, Pictet R . Isolation and characterization of a cloned rat insulin gene. Cell. 1979; 18(2):533-43. DOI: 10.1016/0092-8674(79)90070-9. View

14.

Lomedico P, Rosenthal N, Efstratidadis A, Gilbert W, Kolodner R, Tizard R . The structure and evolution of the two nonallelic rat preproinsulin genes. Cell. 1979; 18(2):545-58. DOI: 10.1016/0092-8674(79)90071-0. View

15.

Houck C, Rinehart F, Schmid C . A ubiquitous family of repeated DNA sequences in the human genome. J Mol Biol. 1979; 132(3):289-306. DOI: 10.1016/0022-2836(79)90261-4. View

16.

Mitchell A, Beauchamp R, Bostock C . A study of sequence homologies in four satellite DNAs of man. J Mol Biol. 1979; 135(1):127-49. DOI: 10.1016/0022-2836(79)90344-9. View

17.

Van der Ploeg L, Konings A, OORT M, Roos D, Bernini L, Flavell R . gamma-beta-Thalassaemia studies showing that deletion of the gamma- and delta-genes influences beta-globin gene expression in man. Nature. 1980; 283(5748):637-42. DOI: 10.1038/283637a0. View

18.

Bell G, Pictet R, Rutter W, Cordell B, Tischer E, Goodman H . Sequence of the human insulin gene. Nature. 1980; 284(5751):26-32. DOI: 10.1038/284026a0. View

19.

Shen C, Maniatis T . The organization of repetitive sequences in a cluster of rabbit beta-like globin genes. Cell. 1980; 19(2):379-91. DOI: 10.1016/0092-8674(80)90512-7. View

20.

Rubin C, Houck C, Deininger P, Friedmann T, Schmid C . Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequences. Nature. 1980; 284(5754):372-4. DOI: 10.1038/284372a0. View