Analysis of Recombination in Mammalian Cells Using SV40 Genome Segments Having Homologous Overlapping Termini

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1980 Jun 25

PMID 6253890

Citations 20

Authors

P Upcroft

B Carter

C Kidson

Affiliations

Soon will be listed here.

Abstract

Segments of SV40 DNA having homologous overlapping termini recombine to produce viable genomes in monkey cells. Frequencies of recombination on either side of a deletion marker are non-random; replication and palindromes do not appear to be essential. Since recombination involves host enzymes, a suitable system has been devised for analysing host cell recombination functions.

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Conversion through homologous recombination of the gene encoding Simian virus 40 115,000-molecular-weight super T antigen to a gene encoding a normal-size large T antigen variant.

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Homologous and nonhomologous recombination in monkey cells.

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